Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation

Hyunil Jo Robert M. Culik Ivan V. Korendovych William F. DeGrado and Feng Gai

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Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation

机译：通过半胱氨酸烷基化选择性地将基于腈的红外探针掺入蛋白质

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The nitrile stretching vibration is increasinglynused as a sensitive infrared probe of local protein environ-nments. However, site-specific incorporation of a nitrilenmoiety into proteins is difficult. Here we show that variousnaromatic nitriles can be easily incorporated into peptidesnand proteins via either thiol alkylation or arylation reaction.

机译：腈拉伸振动越来越多地被用作局部蛋白质环境的敏感红外探针。然而，将腈基位点特异性掺入蛋白质是困难的。在这里我们表明，各种巯基腈可以通过硫醇烷基化或芳基化反应轻松地掺入肽和蛋白质中。

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《Biochemistry》 |2010年第49期|p.10354-10356|共3页
作者
Hyunil Jo Robert M. Culik Ivan V. Korendovych William F. DeGrado and Feng Gai;
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‡Department of Chemistry and §Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia,Pennsylvania 19104, United States.) These authors contributed equally to this work.;

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1. Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation [J] . Jo H, Culik RM, Korendovych IV, Biochemistry . 2010,第49期

机译：通过半胱氨酸烷基化选择性地将基于腈的红外探针掺入蛋白质中
2. Probing acrylamide alkylation sites in cysteine-free proteins by matrix-assisted laser desorption/ionisation time-of-flight [J] . Bordini E., Righetti PG., Hamdan M. Rapid Communications in Mass Spectrometry: RCM . 2000,第10期

机译：通过基质辅助激光解吸/电离飞行时间探测无半胱氨酸蛋白中的丙烯酰胺烷基化位点
3. Probing protein unfolding through monitoring cysteine alkylation by matrix-assisted laser desorption/ionisation mass spectrometry [J] . Galvani M., Righetti PG., Hamdan M. Rapid Communications in Mass Spectrometry: RCM . 2000,第20期

机译：通过基质辅助激光解吸/电离质谱监测半胱氨酸烷基化来探测蛋白质的解链
4. Comparative analysis of cysteine alkylation and the effects on tandem mass spectra and protein identification. [C] . M. J. Raftery ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：半胱氨酸烷基化的比较分析及对串联质谱和蛋白质鉴定的影响。
5. Site-specific incorporation of biochemical and biophysical probes into proteins using expressed protein ligation. [D] . Holford, Mande. 2003

机译：使用表达的蛋白质连接，将生化和生物物理探针位点特异性结合到蛋白质中。
6. Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation [O] . Hyunil Jo, Robert M. Culik, Ivan V. Korendovych, -1

机译：通过半胱氨酸烷基化腈基红外探头的选择性掺入蛋白质
7. Mitochondria-targeting near-infrared ratiometric fluorescent probe for selective imaging of cysteine in orthotopic lung cancer mice [O] . Xiaoyu Zhang, Na He, Yan Huang, 2019

机译：线粒体靶向近红外比率荧光探针，用于在原位肺癌小鼠中的半胱氨酸选择性成像

Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation

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