Novel Hydrophobic Standards for Membrane Protein Molecular Weight Determinations via Sodium Dodecyl Sulfate−Polyacrylamide Gel Electrophoresis

Arianna Rath Vincent G. Nadeau Bradley E. Poulsen Derek P. Ng and Charles M. Deber

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Novel Hydrophobic Standards for Membrane Protein Molecular Weight Determinations via Sodium Dodecyl Sulfate−Polyacrylamide Gel Electrophoresis

机译：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定膜蛋白分子量的新型疏水标准

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Sodium dodecyl sulfate-polyacrylamide gelnelectrophoresis (SDS-PAGE) is a universally employedntechnique that separates proteins on the basis of molecularnweight (MW). However, membrane proteins are known tonsize anomalously on SDS-PAGE calibrated with conven-ntional standards, an issue that complicates interpretation ofnprotein identity, purity, degradation, and/or stoichiometry.nHere we describe the preparation of novel polyleucinenhydrophobic standards for SDS-PAGE that reduce thenaverage deviation of the apparent MW from the formulanMWof natural membrane proteins to 7%versus 20%withncommercially available standards. Our results suggest thatngel calibration with hydrophobic standards may facilitate theninterpretation ofmembrane protein SDS-PAGE experiments.

机译：十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）是一种普遍使用的技术，可根据分子量（MW）分离蛋白质。然而，已知膜蛋白在用常规标准品校准的SDS-PAGE上反常泛化，这个问题使对蛋白同一性，纯度，降解和/或化学计量的解释变得复杂。将天然膜蛋白的分子量从表观MW的平均偏差降低到7％对20％（非商业标准）。我们的结果表明，用疏水性标准物进行凝胶校准可能有助于随后对膜蛋白SDS-PAGE实验的解释。

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《Biochemistry》 |2010年第50期|p.10589-10591|共3页
作者
Arianna Rath Vincent G. Nadeau Bradley E. Poulsen Derek P. Ng and Charles M. Deber;
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‡Division of Molecular Structure and Function, Research Institute, The Hospital for Sick Children, Toronto, Ontario,Canada M5G 1X8, and §Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8;

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1. Novel Hydrophobic Standards for Membrane Protein Molecular Weight Determinations via Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis [J] . Rath A, Nadeau VG, Poulsen BE, Biochemistry . 2010,第50期

机译：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定膜蛋白分子量的新型疏水标准
2. Carbon nanotube-modified sodium dodecyl sulfate–polyacrylamide gel electrophoresis for molecular weight determination of proteins [J] . Meera Parthasarathy, Joyashish Debgupta, Bhalchandra Kakade, Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2011,第2期

机译：碳纳米管修饰的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定蛋白质的分子量
3. Correction factors for membrane protein molecular weight readouts on sodium dodecyl sulfate-polyacrylamide gel electrophoresis [J] . Rath A., Deber C.M. Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2013,第1期

机译：十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上膜蛋白分子量读数的校正因子
4. Protein Profile Euthynnus Affinis Fish Submerse Alum Solution Based on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) [C] . Akhmad Mubarok, Sri Darmawati, Endang T. M. International Conference Postgraduate School Universitas Airlangga . 2018

机译：基于十二烷基硫酸钠聚丙烯酰胺凝胶电泳的蛋白质概况Euthynnus Affinis鱼浸渍液（SDS-PAGE）
5. A NOVEL APPROACH TO PROTEIN SEQUENCING BASED ON PARTIAL SPECIFIC CLEAVAGE OF END-LABELED PROTEINS AND ANALYSIS ON SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS (BIOCHEMISTRY) [D] . JUE, RODNEY ALAN 1984

机译：基于末端特定蛋白质部分裂解的蛋白质序列分离新方法及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（生物化学）分析
6. Analyses of gonococcal lipopolysaccharide in whole-cell lysates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: stable association of lipopolysaccharide with the major outer membrane protein (protein I) of Neisseria gonorrhoeae. [O] . P J Hitchcock 1984

机译：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析全细胞裂解液中的淋球菌脂多糖：脂多糖与淋病奈瑟氏球菌的主要外膜蛋白（蛋白I）稳定缔合。
7. Determination of the Molecular Weights of Gluten Proteins from Ferguson Plots in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis [O] . Zen-ichiro Hamauzu, Yoshiko Mabuchi, Keiko Matsutaka 1982

机译：十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中Ferguson斑块的麸质蛋白分子量的测定
8. Variable Expression of Membrane Proteins among Murine Lymphoblastoid Tumors as Seen with Sodium Dodecyl Sulfate-Polyacrylamide Gradient Gel Electophoresis of (35S) Methionine-Labeled Cell Membranes [R] . Malenka, D. J., Rogan, K. M., Howe, R. C., 1979

机译：（35s）甲硫氨酸标记细胞膜十二烷基硫酸钠 - 聚丙烯酰胺梯度凝胶电泳中小鼠淋巴母细胞瘤中膜蛋白的可变表达

Novel Hydrophobic Standards for Membrane Protein Molecular Weight Determinations via Sodium Dodecyl Sulfate−Polyacrylamide Gel Electrophoresis

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