Replacement of an Electron Transfer Pathway in Cytochrome c Peroxidase with a Surrogate Peptide,

Anna-Maria A. Hays Putnam Young-Tae Lee and David B. Goodin

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Replacement of an Electron Transfer Pathway in Cytochrome c Peroxidase with a Surrogate Peptide,

机译：用替代肽替代细胞色素c过氧化物酶中的电子转移途径，

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A proposed electron transfer pathway in cytochrome c peroxidase was previously excised from the structure by design. The engineered channel mutant was shown to bind peptide surrogates without restoration of cyt c oxidation. Here, we report the 1.6 Å crystal structure of (N-benzimidazole-propionic acid)-Gly-Ala-Ala bound within the engineered channel. The peptide retains many features of the native electron transfer pathway: placement of benzimidazole at the position of the Trp-191 radical, hydrogen bonding to Asp235, and positioning of the C-terminus near the point where wild type CcP makes closest contact to cyt c. The inability of this surrogate pathway to restore function supports proposals that electron transfer requires the Trp-191 radical.

机译：先前通过设计从结构中切除了细胞色素c过氧化物酶中拟议的电子转移途径。该工程通道突变体显示出结合肽替代物而不恢复cyt c氧化。在这里，我们报告了工程通道内结合的（N-苯并咪唑-丙酸）-Gly-Ala-Ala的1.6Å晶体结构。该肽保留了天然电子转移途径的许多特征：将苯并咪唑置于Trp-191自由基的位置，氢键与Asp235的位置以及C端的位置靠近野生型CcP与cyt c最紧密接触的位置。该替代途径无法恢复功能，支持了电子转移需要Trp-191自由基的提议。

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《Biochemistry》 |2009年第1期|p.1-3|共3页
作者
Anna-Maria A. Hays Putnam Young-Tae Lee and David B. Goodin;
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Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037;

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1. Excision of a proposed electron transfer pathway in cytochrome c peroxidase and its replacement by a ligand-binding channel. [J] . Rosenfeld RJ, Hays AM, Musah RA, Protein Science: A Publication of the Protein Society . 2002,第5期

机译：在细胞色素c过氧化物酶中提议的电子转移途径的切除及其被配体结合通道替代。
2. Photoinduced electron transfer between cytochrome c peroxidase (D37K) and Zn-substituted cytochrome c: probing the two-domain binding and reactivity of the peroxidase [J] . Jian S.Zhou, Suong T.Tran, George McLendon Journal of the American Chemical Society . 1997,第2期

机译：细胞色素c过氧化物酶（D37K）与Zn取代的细胞色素c之间的光诱导电子转移：探索过氧化物酶的两个域结合和反应性
3. Trapping of peptide-based surrogates in an artificially created channel of cytochrome c peroxidase. [J] . Hays AM, Gray HB, Goodin DB Protein Science: A Publication of the Protein Society . 2003,第2期

机译：在人工创建的细胞色素C过氧化物酶通道中捕获基于肽的替代物。
4. The membrane-bound C type cytochromes and the interaction between the downhill and uphill electron transfer pathways in the acidophilic chemolitotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans [C] . D. Lemesle-Meunier, G. Brasseur, P. Tron, International Biohydrometallurgy Symposium, IBS-2001 Sep 16-19, 2001, Ouro Preto, Minas Gerais, Brazil . 2001

机译：嗜酸性化学营养亚铁离子氧化细菌氧化亚铁硫杆菌中膜结合的C型细胞色素以及上下电子传递途径之间的相互作用
5. Electron transfer and catalysis in bacterial diheme cytochrome c peroxidases and peroxidase paralogues: Developing a structure function relationship. [D] . Ellis, Katie E. 2013

机译：电子转移和催化细菌双血红素细胞色素C过氧化物酶和过氧化物酶旁系同源物：建立结构功能关系。
6. Replacement of an Electron Transfer Pathway in Cytochrome c Peroxidase with a Surrogate Peptide [O] . Anna-Maria A. Hays Putnam, Young-Tae Lee, David B. Goodin -1

机译：用替代肽替换细胞色素c过氧化物酶中的电子转移途径
7. Excision of a proposed electron transfer pathway in cytochrome c peroxidase and its replacement by a ligand-binding channel [O] . Rosenfeld, Robin J., Hays, Anna-Maria A., Musah, Rabi A., 2002

机译：提议的细胞色素c过氧化物酶电子转移途径的切除及其被配体结合通道替代

Replacement of an Electron Transfer Pathway in Cytochrome c Peroxidase with a Surrogate Peptide,

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