• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >C-Terminal Membrane Spanning Region of Human Heme Oxygenase-1 Mediates a Time-Dependent Complex Formation with Cytochrome P450 Reductase
【24h】

C-Terminal Membrane Spanning Region of Human Heme Oxygenase-1 Mediates a Time-Dependent Complex Formation with Cytochrome P450 Reductase

机译：人血红素加氧酶-1的C末端跨膜区介导与细胞色素P450还原酶的时间依赖性复合物形成。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Heme oxygenase-1 (HO-1) catalyzes the oxidative degradation of heme to biliverdin, carbon monoxide, and free iron in a reaction requiring the interaction of HO-1 with NADPH−cytochrome P450 reductase (CPR). HO-1 is bound to the endoplasmic reticulum by 23 C-terminal amino acids; however, a soluble HO-1 (sHO-1) lacking this membrane spanning region has been extensively studied. The goal of this project was to characterize the effect of the C-terminal hydrophobic domain on formation of the HO-1/CPR complex. Full-length HO-1 was shown to exhibit higher reaction rates than sHO-1, particularly at subsaturating CPR, indicating that the C-terminal region influences HO-1 binding to CPR. The increased activity of HO-1 was attributable to a time-dependent formation of a low Km HO-1/CPR complex that was not seen with sHO1. Gel filtration analysis confirmed the formation of multiple high molecular weight complexes in the presence and absence of the synthetic lipid dilauroylphosphatidylcholine (DLPC). However, the largest complex appeared following a 2 h incubation of HO-1 and CPR in DLPC, suggesting that the C-terminal region was required for the high-affinity HO-1/CPR complex formation and membrane incorporation. These data demonstrate that the C-terminal region of HO-1 influenced complex formation and ultimately its affinity for CPR.
机译：在需要HO-1与NADPH-细胞色素P450还原酶（CPR）相互作用的反应中，血红素加氧酶-1（HO-1）催化血红素氧化降解为Biliverdin，一氧化碳和游离铁。 HO-1通过23个C末端氨基酸与内质网结合；但是，已经广泛研究了缺少该跨膜区域的可溶性HO-1（sHO-1）。该项目的目的是表征C端疏水域对HO-1 / CPR复合物形成的影响。全长HO-1已显示出比sHO-1更高的反应速率，特别是在CPR亚饱和时，表明C末端区域影响HO-1与CPR的结合。 HO-1活性的增加归因于时间依赖性的低Km HO-1 / CPR复合物的形成，而sHO1则没有。凝胶过滤分析证实在存在和不存在合成脂质二月桂酰磷脂酰胆碱（DLPC）的情况下形成多种高分子量复合物。然而，最大的复合物出现在DLPC中HO-1和CPR孵育2小时后，这表明高亲和力HO-1 / CPR复合物形成和膜掺入需要C端区域。这些数据表明HO-1的C末端区域影响复合物的形成，并最终影响其对CPR的亲和力。

著录项

  • 来源
    《Biochemistry》 |2009年第1期|p.190-197|共8页
  • 作者

    Warren J. Huber III Brittni A. Scruggs and Wayne L. Backes;

  • 作者单位

    Department of Pharmacology and The Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号