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首页> 外文期刊>Biochemistry >Integration Host Factor (IHF) Dictates the Structure of Polyamine-DNA Condensates: Implications for the Role of IHF in the Compaction of Bacterial Chromatin
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Integration Host Factor (IHF) Dictates the Structure of Polyamine-DNA Condensates: Implications for the Role of IHF in the Compaction of Bacterial Chromatin

机译：整合宿主因子（IHF）决定多胺-DNA冷凝物的结构：对IHF在细菌染色质压实中的作用的暗示

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摘要

Integration host factor (IHF), a nucleoid-associated protein in bacterial cells, is implicated in annumber of chromosomal functions including DNA compaction. IHF binds to all duplex DNA with micromolarnaffinity and at sequence-specific sites with much higher affinity. IHF is known to induce sharp bends in thenhelical axis of DNA in both modes of binding, but the role of IHF in controlling DNA condensation withinnbacterial cells has remained undetermined. Here we demonstrate that IHF influences the morphology of DNAncondensed by polyamines in vitro. In the absence of IHF, spermidine and spermine condense DNA primarilyninto toroidal structures, whereas in the presence of IHF, polyamines condense DNA primarily into rodlikenstructures. Computer simulations of DNA condensation in the absence and presence of IHF binding lend supportnto our model in which DNA bending proteins, such as IHF and HU, promote the condensation of DNA intonrodlike structures by providing the free energy necessary to bend DNA at the ends of linear bundles of condensednDNA. We propose that a common function of IHF and HU in bacterial cells is to facilitate DNA organizationnin the nucleoid by the introduction of sharp bends in chromosomal DNA.
机译：整合宿主因子（IHF）是细菌细胞中与核苷酸相关的蛋白质，与许多染色体功能有关，包括DNA压缩。 IHF以微摩尔亲和力和序列特异性位点以更高的亲和力与所有双链DNA结合。众所周知，IHF会以两种结合方式在DNA的螺旋轴上引起急剧的弯曲，但是IHF在控制细菌细胞内DNA缩合中的作用尚未确定。在这里，我们证明了IHF影响体外多胺缩合的DNAn的形态。在没有IHF的情况下，亚精胺和亚精胺将DNA主要缩合为环状结构，而在IHF的情况下，多胺主要将DNA缩合为棒状结构。在不存在IHF结合的情况下进行DNA缩合的计算机模拟为我们的模型提供了支持，在模型中，DNA弯曲蛋白（例如IHF和HU）通过提供线性末端处弯曲DNA所需的自由能来促进DNA扁节状结构的凝结。一束浓缩的DNA。我们提出，细菌细胞中IHF和HU的共同功能是通过在染色体DNA中引入急剧弯曲来促进核苷酸中的DNA组织。

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