Molecular Mechanism for the Denaturation of Proteins by Urea

Jorge Almarza Luis Rincon Ali Bahsas and Francisco Brito

首页> 外文期刊>Biochemistry >Molecular Mechanism for the Denaturation of Proteins by Urea

【24h】

Molecular Mechanism for the Denaturation of Proteins by Urea

机译：尿素使蛋白质变性的分子机理

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Understanding protein-solute interactions is one of the sizable challenges of protein chemistry;ntherefore, numerous experimental studies have attempted to explain the mechanism by which proteins unfoldnin aqueous urea solutions.On the basis of kinetic evidence at low urea concentrations,n1nHNMRspectroscopicnanalysis, andmolecular orbital calculations, we propose amechanisticmodel for the denaturation ofRNaseAnin urea. Our results support a direct interaction between urea and protonated histidine as the initial step fornprotein inactivation followed by hydrogen bond formation with polar residues, and the breaking ofnhydrophobic collapse as the final steps for protein denaturation.With the proposedmodel, we can rationalizenapparently conflicting results in the literature about the mechanism of protein denaturation with urea.

机译：理解蛋白质-溶质相互作用是蛋白质化学领域的重大挑战之一；因此，许多实验研究试图解释蛋白质在尿素水溶液中展开的机理。在低尿素浓度的动力学证据，n1nHNMR光谱分析和分子轨道计算的基础上，我们提出了RNaseAnin尿素变性的机理模型。我们的研究结果支持尿素和质子化组氨酸之间的直接相互作用，作为蛋白质失活的第一步，然后形成带有极性残基的氢键，打破疏水性破坏作为蛋白质变性的最终步骤。关于尿素蛋白质变性机理的文献。

著录项

来源
《Biochemistry》 |2009年第32期|p.7608-7613|共6页
作者
Jorge Almarza Luis Rincon Ali Bahsas and Francisco Brito;
展开▼
作者单位

‡Laboratorio de Genu0001 etica y Quı ´mica Celular, Departamento de Biologı ´a, Facultad de Ciencias, Universidad de Los Andes (ULA),Mu0001 erida 5101,Venezuela,§Departamento deQuı ´mica, Facultad deCiencias,Universidad deLosAndes (ULA),Merida 5101,Venezuela,and ) Departamento de Quı ´mica, Nu0001 ucleo de Quı ´mica Avanzada, Universidad Nacional Experimental del Tu0001 achira (UNET), SanCristu0001 obal, Venezuela;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Exploring the Counteracting Mechanism of Trehalose on Urea Conferred Protein Denaturation: A Molecular Dynamics Simulation Study [J] . Paul Subrata, Paul Sandip The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical . 2015,第30期

机译：探索海藻糖对尿素赋予蛋白变性的抑制机理：分子动力学模拟研究
2. A naturally occurring protective system in urea-rich cells: mechanism of osmolyte protection of proteins against urea denaturation [J] . Wang A, Bolen DW Biochemistry . 1997,第30期

机译：富含尿素的细胞中天然存在的保护系统：渗透蛋白保护蛋白质抵抗尿素变性的机制
3. Mechanisms of amphipathic helical peptide denaturation by guanidinium chloride and urea: A molecular dynamics simulation study [J] . Mehrnejad F., Khadem-Maaref M., Ghahremanpour M.M., Journal of Computer-Aided Molecular Design . 2010,第10期

机译：氯化胍和尿素使两亲性螺旋肽变性的机理：分子动力学模拟研究
4. In-Silico Structural Analysis of SoxF Protein Through Molecular Modelling and Protein-Protein Docking from Hydrogenophilus thermoluteolus: An Approach to Understand the Molecular Mechanism of Thiosulfate Oxidation [C] . Sujay Ray, Angshuman Bagchi International Conference on Information Systems Design and Intelligence Applications . 2015

机译：SOXF蛋白通过分子建模和蛋白质 - 蛋白蛋白对催泪蛋白的硅蛋白质结构分析：一种了解硫代硫酸盐氧化分子机制的方法
5. Molecular determinants of the thermal expansivity of proteins and volumetric changes upon protein denaturation. [D] . Pandharipande, Pranav. 2016

机译：蛋白质热膨胀性和蛋白质变性后体积变化的分子决定因素。
6. Mechanism of Protein Denaturation: Partial Unfolding of the P22 Coat Protein I-Domain by Urea Binding [O] . Rebecca L. Newcomer, LaTasha C.R. Fraser, Carolyn M. Teschke, 2015

机译：蛋白质变性的机制：尿素结合的P22外壳蛋白I域的部分展开。
7. Molecular Mechanism of Urea-Induced Protein Denaturation [O] . Lee Soyoung, Shek Yuen Lai, Chalikian Tigran V. 2010

机译：尿素诱导蛋白质变性的分子机理

Molecular Mechanism for the Denaturation of Proteins by Urea

摘要

著录项

相似文献

相关主题

期刊订阅