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首页> 外文期刊>Biochemistry >Effects of Osmolytes on the SLN1-YPD1-SSK1 Phosphorelay System from Saccharomyces cerevisiae
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Effects of Osmolytes on the SLN1-YPD1-SSK1 Phosphorelay System from Saccharomyces cerevisiae

机译：渗透液对酿酒酵母SLN1-YPD1-SSK1磷酸化系统的影响

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摘要

The multistep His-Asp phosphorelay system in Saccharomyces cerevisiae allows cells to adapt tonosmotic, oxidative, and other environmental stresses. The pathway consists of a hybrid histidine kinase SLN1,na histidine-containing phosphotransfer (HPt) protein YPD1, and two response regulator proteins, SSK1 andnSKN7. Under nonosmotic stress conditions, the SLN1 sensor kinase is active, and phosphoryl groups arenshuttled through YPD1 to SSK1, therefore maintaining the response regulator protein in a constitutivelynphosphorylated state. The cellular response to hyperosmotic stress involves rapid efflux of water and changesnin intracellular ion and osmolyte concentration. In this study, we examined the individual and combinedneffects of NaCl and glycerol on phosphotransfer rates within the SLN1-YPD1-SSK1 phosphorelay. Thenresults show that the combined effects of glycerol and NaCl on the phosphotransfer reaction rates arendifferent from the individual effects of glycerol and NaCl. The combinatory effect is likely more representativenof the in vivo changes that occur during hyperosmotic stress. In addition, the effect of osmolyte concentrationnon the half-life of the phosphorylated SSK1 receiver domain in the presence/absence of YPD1 was evaluated.nOur findings demonstrate that increasing osmolyte concentrations negatively affect the YPD1 3 SSK1∼Pninteraction, thereby facilitating dephosphorylation of SSK1 and activating the HOG1 MAP kinase cascade.nIn contrast, at the highest osmolyte concentrations, reflective of the osmoadaptation phase of the signalingnpathway, the kinetics of the phosphorelay favor production of SSK1∼P and inhibition of theHOG1 pathway.
机译：酿酒酵母中的多步His-Asp磷酸化沉积系统使细胞能够适应菌突性，氧化性和其他环境胁迫。该途径由杂交组氨酸激酶SLN1，含组氨酸的磷酸转移（HPt）蛋白YPD1和两个响应调节蛋白SSK1和nSKN7组成。在非渗透胁迫条件下，SLN1传感器激酶是有活性的，磷酸基通过YPD1穿梭到SSK1，因此将响应调节蛋白维持在组成型磷酸化状态。细胞对高渗胁迫的反应涉及水的快速流出以及细胞内离子和渗透液浓度的变化。在这项研究中，我们检查了NaCl和甘油对SLN1-YPD1-SSK1磷光体内磷转移速率的个体和联合作用。结果表明，甘油和氯化钠对磷酸转移反应速率的综合影响与甘油和氯化钠的个别影响没有区别。组合作用可能更能代表高渗应激期间发生的体内变化。此外，评估了渗透液浓度对存在/不存在YPD1时磷酸化SSK1受体结构域半衰期的影响.n我们的研究结果表明，渗透液浓度的增加会对YPD1 3 SSK1〜Pn相互作用产生负面影响，从而促进SSK1和SSK1的去磷酸化相比之下，在最高渗透压浓度下，反映信号通路的渗透适应阶段，磷矿的动力学有利于SSK1〜P的产生和HOG1通路的抑制。

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