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Butanol Production from Leftover Beverages and Sport Drinks

机译:剩余饮料和运动饮料生产丁醇

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摘要

The aim of this paper is twofold: (1) to identify an alternative disposal process for the industry of high-sugar-content beverages (HSCBs) and (2) to contribute to the study of butanol production from non-edible feedstocks. HSCBs were used as a renewable feedstock to produce butanol by Clostridium acetobutylicum DSM 792. Four types of commercial beverages were investigated: fruit juices (pineapple and pear), syrups (lemon and almond), soft drinks, and a sport drink. They contained high levels of sugar—between 50 and 1,000 g/L—mainly glucose, fructose, and sucrose. These sugars were also tested individually and in a mixture. The batch fermentation tests were characterized in terms of metabolite and cell production, and sugar conversion. C. acetobutylicum was able to grow on HSCBs supplemented with yeast extract and minerals. The tests on the sugars (alone or mixed) highlighted the predisposition of C. acetobutylicum to metabolize them and to produce butanol. The production rate of butanol (rBMAX) increased in the following order: glucose, fructose, and sucrose. The HSCB pre-hydrolysis improved the fermentation performance in terms of solvent production and sugar conversion (almost 100 %) because sucrose has been hydrolyzed. Under optimized conditions the butanol concentration and yield were—13.3 g/L, 0.16 g/g for pineapple juice; 12.8 g/L, 0.14 g/g for pear juice; 13.2 g/L, 0.19 g/g for lemon syrup; 13.8 g/L, 0.14 g/g for almond syrup; 13–14 g/L, 0.17–0.18 g/g for commercial soft drinks, and 5.9 g/L, 0.11 g/g for sport drinks. Among the beverages tested, the sport drinks gave the maximum butanol production rate of 12 g/L h.
机译:本文的目的有两个:(1)为高糖含量饮料(HSCBs)行业确定一种替代处置方法;(2)为研究非食用原料生产丁醇做出贡献。 HSCB被丙酮丁醇梭菌DSM 792用作可再生原料生产丁醇。研究了四种类型的商业饮料:果汁(菠萝和梨),糖浆(柠檬和杏仁),软饮料和运动饮料。它们含有高含量的糖-在50至1,000 g / L之间-主要是葡萄糖,果糖和蔗糖。还对这些糖进行了单独测试和混合测试。分批发酵测试以代谢物和细胞产生以及糖转化为特征。丙酮丁醇梭菌能够在补充了酵母提取物和矿物质的HSCB上生长。对糖(单独或混合)的测试强调了丙酮丁醇梭菌易于代谢和产生丁醇的倾向。丁醇(rBMAX)的生产速率按以下顺序增加:葡萄糖,果糖和蔗糖。由于蔗糖已被水解,因此HSCB预水解改善了溶剂生产和糖转化率(几乎100%)方面的发酵性能。在最佳条件下,丁醇的浓度和产量为-13.3 g / L,菠萝汁为0.16 g / g; 12.8克/升,梨汁0.14克/克; 13.2克/升,柠檬糖浆0.19克/克; 13.8 g / L,杏仁糖浆0.14 g / g; 13–14 g / L,商业软饮料为0.17–0.18 g / g,运动饮料为5.9 g / L,0.11 g / g。在测试的饮料中,运动饮料的最大丁醇生产率为12 g / L·h。

著录项

  • 来源
    《Bioenergy research》 |2015年第1期|369-379|共11页
  • 作者单位

    1.Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Università degli Studi di Napoli Federico II P.le V. Tecchio 80 80125 Naples Italy;

    1.Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Università degli Studi di Napoli Federico II P.le V. Tecchio 80 80125 Naples Italy;

    2.Dipartimento di Scienze Chimiche Università degli Studi di Napoli Federico II Complesso Universitario di Monte Sant’Angelo 80126 Naples Italy;

    1.Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Università degli Studi di Napoli Federico II P.le V. Tecchio 80 80125 Naples Italy 3.Wageningen University and Research Centre Droevendaalsesteeg 1 P.O. Box 8129 6708 PB Wageningen The Netherlands;

    1.Dipartimento di Ingegneria Chimica dei Materiali e della Produzione Industriale Università degli Studi di Napoli Federico II P.le V. Tecchio 80 80125 Naples Italy;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Butanol Clostridium acetobutylicum Fructose Sucrose High-sugar-content beverage;

    机译:丁醇丙酮丁醇梭菌果糖蔗糖高糖饮料;

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