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首页> 外文期刊>Bioenergy research >Lowland Switchgrass Plants in Populations Set Completely Outcrossed Seeds Under Field Conditions as Assessed with SSR Markers
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Lowland Switchgrass Plants in Populations Set Completely Outcrossed Seeds Under Field Conditions as Assessed with SSR Markers

机译：根据SSR标记评估，田间条件下种群中的低地柳枝植物完全交交了种子

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摘要

Switchgrass has long been characterized as an outcrossing species. However, mating behavior of plants in populations grown in the field allowing open pollination has not been documented. Accordingly, the objectives of this study were to determine the fertilization mode (i.e., selfing vs. outcrossing) of two self-compatible plants and to assess the mating behavior variability of lowland switchgrass genotypes in populations under field conditions. In Experiment I, two self-compatible genotypes ‘NL94 LYE 16 × 13’ and ‘SL93 7 × 15’ along with two populations were planted with two replications on the OSU Agronomy Research Farm, Stillwater, OK. Sixty-four progeny derived from half-sib seeds of each genotype per replication per year were genotyped with 4 to 20 simple sequence repeat (SSR) markers. In both 2010 and 2011, all progeny plants of the two parents were completely outcrossed exhibiting 100 % self-incompatibility. In Experiment II, two genetically narrow-based (NL94 C2-3 and SL93 C2-3), each having five parents, and two broad-based (NL94 C3 and SL93 C3), each comprising 26 parents, switchgrass populations with three replications were field established at the OSU Cimarron Valley Research Station, Perkins, OK. The DNA samples were isolated from 1700 open-pollinated progeny of 62 seed parents in 2010 and 773 progeny of 42 parents in 2011. Among all the progeny genotyped with eight to 16 SSR markers, only one was identified as a selfed progeny, indicating very little variability in outcrossing behavior under the field conditions. The identification of specific genotypes like NL94 LYE 16 × 13 and SL93 7 × 15 which are self-incompatible in the open field but self-compatible under the controlled conditions potentially enables efficient production of F1 hybrid seed in switchgrass.
机译：柳枝long长期以来被描述为异种。但是，尚未记录在田间生长的种群中允许开放授粉的植物交配行为。因此，本研究的目的是确定两种自交植物的受精模式（即自交与异交），并评估田间条件下低地柳枝switch基因型在种群中的交配行为变异性。在实验一中，在俄克拉荷马州斯蒂尔沃特市的俄勒冈州立大学农学研究农场种植了两个自我兼容的基因型“ NL94 LYE 16×13”和“ SL93 7×15”以及两个种群。每年每复制一次，从每种基因型的半同胞种子衍生的六十四个后代，用4至20个简单序列重复（SSR）标记进行基因分型。在2010年和2011年，两个亲本的所有后代植物都完全杂交，表现出100％的自我不相容性。在实验II中，两个遗传基础较窄的（NL94 C2-3和SL93 C2-3），每个都有五个亲本，两个遗传基础较宽的（NL94 C3和SL93 C3），每个都有26个亲本，柳枝population种群具有三个重复。俄克拉荷马州珀金斯市的OSU Cimarron山谷研究站建立了该油田。 DNA样本是从2010年的62个种子亲本的1700个开放授粉后代和2011年的42个亲本的773个后代中分离得到的。在所有具有8至16个SSR标记的基因型中，只有一个被鉴定为自交后代，表明很少野外条件下越野行为的变异性。鉴定特定基因型，例如NL94 LYE 16××13和SL93 7××15，它们在旷野中不相容，但在受控条件下具有自相容性，因此有可能在柳枝efficient中高效生产F1杂种。

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  • 来源
    《Bioenergy research》 |2014年第1期|253-259|共7页
  • 作者

    Linglong Liu; Shuiyi Lu Thames; Yanqi Wu;

  • 作者单位

    1.Department of Plant and Soil Sciences Oklahoma State University Stillwater OK 74078 USA 2.National Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research Center Nanjing Agricultural University Nanjing 210095 China;

    1.Department of Plant and Soil Sciences Oklahoma State University Stillwater OK 74078 USA;

    1.Department of Plant and Soil Sciences Oklahoma State University Stillwater OK 74078 USA;

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  • 关键词

    Self-incompatibility (SI) Outcrossing Simple sequence repeat (SSR) Hybrid seed Switchgrass;

    机译：自交不亲和（SI）杂交简单序列重复（SSR）杂交种子柳枝Switch;

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