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首页> 外文期刊>Bioenergy research >Transcriptome and Gene Expression Analysis of an Oleaginous Diatom Under Different Salinity Conditions
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Transcriptome and Gene Expression Analysis of an Oleaginous Diatom Under Different Salinity Conditions

机译：不同盐度条件下油质硅藻的转录组和基因表达分析

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摘要

Diatoms constitute a remarkably diverse and attractive group of microalgae, serving as the main primary producers in many ecosystems and a potential source of renewable biofuel. The enhancement of lipid production in diatoms has been achieved by the optimization of culture conditions, such as temperature, salinity, and nutrient starvation. In this study, we performed Illumina sequencing and the de novo transcriptome assembly of an oleaginous diatom, Nitzschia sp., which produces up to 50 % oil by weight under defined conditions. High-quality reads were assembled into 28,117 isogenes and then subjected to BLAST alignment, Gene Ontology annotation, and KEGG Orthology annotation. The majority of genes and pathways related to cell wall formation and lipid biosynthesis were identified by these analyses. In addition, elevated salinity was found to increase the total lipid content of Nitzschia sp. For a better understanding of the molecular mechanisms regulating this phenomenon, transcriptome profiles under different conditions of salinity were compared to examine how the metabolic flux was channeled to increase the biosynthesis of triacylglycerols. As expected, a subset of genes involved in lipid biosynthesis was up-regulated under salinity stress. Meanwhile, carbon and nitrogen metabolism genes were also significantly affected, indicating a diversion of metabolic pathways. The data we generated here enrich the genomic resources available for non-model algae and provide insights into the mechanisms of lipid accumulation in microalgae.
机译：硅藻构成了非常多样化和有吸引力的微藻群，是许多生态系统中的主要初级生产者，也是可再生生物燃料的潜在来源。通过优化培养条件（例如温度，盐度和营养饥饿）可以实现硅藻中脂质产量的增加。在这项研究中，我们对含油硅藻Nitzschia sp。进行了Illumina测序和从头转录组组装，在规定的条件下，该硅藻产生了高达50％的重量油。高质量的读段被组装成28,117个同基因，然后进行BLAST比对，基因本体论注释和KEGG Orthology注释。这些分析确定了与细胞壁形成和脂质生物合成有关的大多数基因和途径。另外，发现盐度升高会增加Nitzschia sp。的总脂质含量。为了更好地了解调节这种现象的分子机制，比较了不同盐度条件下的转录组图谱，以检查如何引导代谢通量以增加三酰甘油的生物合成。不出所料，在盐分胁迫下，参与脂质生物合成的基因的一个子集被上调。同时，碳和氮代谢基因也受到显着影响，表明代谢途径的转移。我们在此处生成的数据丰富了非模型藻类可用的基因组资源，并提供了对微藻类中脂质积累机制的见解。

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  • 来源
    《Bioenergy research》 |2014年第1期|192-205|共14页
  • 作者

    Ruo-lin Cheng; Jia Feng; Bing-Xin Zhang; Yun Huang; Jun Cheng; Chuan-Xi Zhang;

  • 作者单位

    1.College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 China;

    2.State Key Laboratory of Clean Energy Utilization Zhejiang University Hangzhou 310027 China;

    1.College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 China;

    2.State Key Laboratory of Clean Energy Utilization Zhejiang University Hangzhou 310027 China;

    2.State Key Laboratory of Clean Energy Utilization Zhejiang University Hangzhou 310027 China;

    1.College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Diatom Differential expression Freshwater Nitzschia Seawater Transcriptome;

    机译：硅藻差异表达淡水棘海水转录组。;

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