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Cellulase and Xylanase Expression in Response to Different pH Levels of Penicillium echinulatum S1M29 Medium

机译:纤维素酶和木聚糖酶表达对不同pH值的青霉菌S1M29培养基的响应

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摘要

The effect of pH on the production of cellulases and xylanases by Penicillium echinulatum S1M29 was evaluated in a shake flask and in a bioreactor. To control the pH in a shake flask, a buffer made with citric acid and disodium phosphate was used. The buffer was capable of maintaining the culture pH values for the first 48 h. In the bioreactor, the pH was controlled automatically by the addition of NaOH and H2SO4. In the shake flask, the highest activities of xylanases (18.5 IU/mL) and endoglucanases (8.2 IU/mL), as well as the highest filter paper activity (FPA) (0.9 IU/mL), were obtained at initial pH values of between 6.0 and 7.0. In the bioreactor, the highest activities of these enzymes were obtained in a pH range of 5.5 to 6.5. Different isoforms of the endoglucanases were found in the various cultures depending on the pH. More acidic pH ranges favored the production of β-glucosidases in both the shake flask and the bioreactor.
机译:在摇瓶和生物反应器中评估了pH对棘皮青霉S1M29产生纤维素酶和木聚糖酶的影响。为了控制摇瓶中的pH,使用了由柠檬酸和磷酸二钠制成的缓冲液。缓冲液能够在最初的48小时内保持培养液的pH值。在生物反应器中,通过添加NaOH和H2SO4自动控制pH。在摇瓶中,木聚糖酶(18.5 IU / mL)和内切葡聚糖酶(8.2 IU / mL)的最高活性以及滤纸活性(FPA)(0.9IU / mL)的最高活性在6.0和7.0之间。在生物反应器中,在5.5至6.5的pH范围内获得了这些酶的最高活性。取决于pH,在各种培养物中发现了内切葡聚糖酶的不同同工型。酸性更大的pH范围有利于在摇瓶和生物反应器中产生β-葡糖苷酶。

著录项

  • 来源
    《Bioenergy research》 |2014年第1期|60-67|共8页
  • 作者单位

    1.Enzymes and Biomass Laboratory Institute of Biotechnology University of Caxias do Sul Rua Francisco Getúlio Vargas 1130 95070-560 Caxias do Sul RS Brazil;

    1.Enzymes and Biomass Laboratory Institute of Biotechnology University of Caxias do Sul Rua Francisco Getúlio Vargas 1130 95070-560 Caxias do Sul RS Brazil;

    1.Enzymes and Biomass Laboratory Institute of Biotechnology University of Caxias do Sul Rua Francisco Getúlio Vargas 1130 95070-560 Caxias do Sul RS Brazil;

    1.Enzymes and Biomass Laboratory Institute of Biotechnology University of Caxias do Sul Rua Francisco Getúlio Vargas 1130 95070-560 Caxias do Sul RS Brazil;

    1.Enzymes and Biomass Laboratory Institute of Biotechnology University of Caxias do Sul Rua Francisco Getúlio Vargas 1130 95070-560 Caxias do Sul RS Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cellulases Xylanases pH Shake flask Bioreactor Zymogram;

    机译:纤维素酶木聚糖酶pH摇瓶生物反应器波形图;

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