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首页> 外文期刊>Biofouling >Inhibitory effect of norharmane on Serratia marcescens NJ01 quorum sensing-mediated virulence factors and biofilm formation
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Inhibitory effect of norharmane on Serratia marcescens NJ01 quorum sensing-mediated virulence factors and biofilm formation

机译：Norharmane对Serratia marcescens NJ01 Quorum感应介导的毒力因子和生物膜形成的抑制作用

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摘要

Serratia marcescens NJ01, a Gram-negative bacterium, can infect tomato leaves and cause chlorosis and wilting. The present study evaluated the quorum sensing (QS) and biofilm inhibitory effects of seven carboline compounds against S. marcescens NJ01 at 20 mu g ml(-1), and subsequently focused the study on norharmane as this had the best inhibitory activity. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis confirmed the down-regulation of QS and biofilm related genes bsmA, bsmB, fimA, fimC, flhD, pigA, pigC and shlA on exposure to norharmane. Fourier-Transform Infrared Spectroscopy (FT-IR) analysis showed a reduction in the major components of the exopolysaccharide (EPS) matrix such as nucleic acids, proteins and fatty acids, which are involved in forming the tertiary structure of biofilms. Norharmane exposure also enhanced the susceptibility of the biofilm to ofloxacin. Hence, norharmane has the potential for use as an antibiotic adjuvant to enhance the efficacy of conventional antibiotics to reduce pathogenic bacterial infections.
机译：Serratia marcescens NJ01，一种革兰氏阴性细菌，可以感染番茄叶并引起氯化和萎氏。本研究评估了七种蛋白质化合物在20μgmml（-1）上对S. marcescens NJ01的仲裁感测（Qs）和生物膜抑制作用，随后将研究重点关注诺拉渣，因为这具有最佳的抑制活性。定量实时聚合酶链反应（QRT-PCR）分析证实了QS和生物膜相关基因BSMA，BSMB，FIMA，FIMC，FLHD，PIGA，PIGC和SHLA暴露于Norharmane的下调。傅立叶变换红外光谱（FT-IR）分析显示出脱核酸（EPS）基质的主要组分如核酸，蛋白质和脂肪酸，其参与形成生物膜的三级结构。诺哈林暴露还提高了生物膜对氧氟沙星的敏感性。因此，诺希兰具有用作抗生素佐剂的可能性，以增强常规抗生素以降低致病细菌感染的功效。

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  • 来源
    《Biofouling》 |2021年第2期|145-160|共16页
  • 作者

    Luo Huai-Zhi; Zhou Jin-Wei; Sun Bing; Jiang Huan; Tang Shi; Jia Ai-Qun;

  • 作者单位

    Nanjing Univ Sci & Technol Sch Environm & Biol Engn Nanjing Peoples R China|Hainan Univ Engn Res Ctr Utilizat Trop Polysaccharide Resourc Sch Life & Pharmaceut Sci Minist Educ Haikou Hainan Peoples R China;

    Xuzhou Univ Technol Sch Food Biol Engn Xuzhou Jiangsu Peoples R China;

    Nanjing Univ Sci & Technol Sch Environm & Biol Engn Nanjing Peoples R China;

    Nanjing Univ Sci & Technol Sch Environm & Biol Engn Nanjing Peoples R China;

    Hainan Univ Engn Res Ctr Utilizat Trop Polysaccharide Resourc Sch Life & Pharmaceut Sci Minist Educ Haikou Hainan Peoples R China;

    Hainan Univ Engn Res Ctr Utilizat Trop Polysaccharide Resourc Sch Life & Pharmaceut Sci Minist Educ Haikou Hainan Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Norharmane; carboline derivatives; Serratia marcescens; quorum sensing; quorum sensing inhibitors; anti-biofilm;

    机译：诺哈兰;蛋白质衍生物;Serratia marcescens;Quorum感测;仲裁感测抑制剂;抗生物膜;

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