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首页> 外文期刊>Biological Trace Element Research >Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF
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Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF

机译：SRXRF揭示的铜吸收及其对Commelina communis根生长区金属分布的影响

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摘要

To explore the copper uptake mechanisms by the Cu-tolerant plant Commelina communis, the contents of Cu and other metals (including Fe, Zn, and Mn) in roots were detected using atomic absorption spectrometer under transporter inhibitors, partial element deficiency, or Cu excess treatments, while distribution characters of Cu and other metals in root growth zones were investigated by synchrotron radiation X-ray fluorescence spectroscopy (SRXRF). Cu uptake was inhibited by the uncoupler DNP and P-type ATPase inhibitor Na3VO4, not by the Ca2+ ion channel inhibitor LaCl3, suggesting that Cu could probably be assimilated actively by root and be related with P-type ATPase, but not through Ca2+ ion channel. Fe or Zn deficiency could enhance Cu uptake, while 100 μM Cu inhibited Fe, Zn, and Mn accumulation in roots significantly. Metal distribution under 100 μM Cu treatment was investigated by SRXRF. High level of Cu was found in the root meristem, and higher Cu concentrations were observed in the vascular cylinder than those in the endodermis, further demonstrating the initiative Cu transport in the root of C. communis. Under excess Cu stress, most Fe was located in the epidermis, and Fe concentrations in the endodermis were higher than those in the vascular cylinder, suggesting Cu and Fe competition not only in the epidermal cells but also for the intercellular and intracellular transport in roots. Zn was present in the meristem and the vascular cylinder similar to Cu. Cu and Zn showed a similar pattern. Mn behaves as Zn does, but not like Fe.
机译：为了探索耐铜植物Commelina communis对铜的吸收机制，在转运抑制剂，部分元素缺乏或铜过量的情况下，使用原子吸收光谱仪检测了根中的铜和其他金属（包括铁，锌和锰）的含量。通过同步辐射X射线荧光光谱法（SRXRF）研究了根处理中Cu和其他金属的分布特征。铜的吸收受解偶联剂DNP和P型ATP酶抑制剂Na 3 VO 4 的抑制，而不受Ca 2 + 离子通道抑制剂LaCl的抑制 3 ，表明Cu可能被根主动吸收并与P型ATPase相关，但与Ca 2 + 离子通道无关。铁或锌缺乏会增加铜的吸收，而100μM的铜会明显抑制根中铁，锌和锰的积累。通过SRXRF研究了100μMCu处理下的金属分布。在根分生组织中发现了高水平的铜，并且在血管圆柱中观察到的铜浓度高于内胚层中的铜浓度，进一步证明了铜在根结线虫根中的主动运输。在过量的铜胁迫下，大多数铁位于表皮中，而表皮中的铁浓度高于维管束中的铁浓度，这表明铜和铁的竞争不仅在表皮细胞中，而且在根部的细胞间和细胞内运输中都存在竞争。锌与铜相似，存在于分生组织和血管圆柱中。 Cu和Zn显示出相似的图案。 Mn的行为与Zn相同，但与Fe不同。

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