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首页> 外文期刊>Biological Trace Element Research >Trigonella foenum-graecum (Fenugreek) Protects Against Selenite-Induced Oxidative Stress in Experimental Cataractogenesis
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Trigonella foenum-graecum (Fenugreek) Protects Against Selenite-Induced Oxidative Stress in Experimental Cataractogenesis

机译：胡芦巴胡芦巴（胡芦巴）在实验性白内障发生中保护免受亚硒酸诱导的氧化应激。

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摘要

Cataract is the opacification in eye lens and leads to 50% of blindness worldwide. The present study was undertaken to evaluate the anticataract potential of Trigonella foenum-graecum Linn seeds (fenugreek) in selenite-induced in vitro and in vivo cataract. In vitro enucleated rat lenses were maintained in organ culture containing Dulbecco’s modified Eagles medium (DMEM) alone or in addition with 100 µM selenite and served as the normal and control groups, respectively. For the test group, the medium was supplemented with selenite and T. foenum-graecum aqueous extract. The lenses were incubated for 24 h at 37°C. After incubation, the lenses were processed for the estimation of reduced glutathione (GSH), lipid peroxidation product (malondialdehyde), and the antioxidant enzymes. In vivo selenite cataract was induced in 9-day-old rats by subcutaneous injection of sodium selenite (25 µmol/kg body weight). Animals in the test group were injected with different doses of aqueous extract of T. foenum-graecum 4 h before the selenite challenge. A fall in GSH and a rise in malondialdehyde levels were observed in control as compared to normal lenses. T. foenum-graecum significantly (P < 0.01) restored glutathione and decreased malondialdehyde levels. A significant restoration in the activities of antioxidant enzymes such as superoxide dismutase (P < 0.01), catalase, (P < 0.01), glutathione peroxidase (P < 0.01), and glutathione-S-transferase (P < 0.01) was observed in the T. foenum-graecum supplemented group as compared to control. In vivo, none of the eyes was found with nuclear cataract in treated group as opposed to 72.5% in the control group. T. foenum-graecum protects against experimental cataract by virtue of its antioxidant properties. Further studies are warranted to explore its role in human cataract.
机译：白内障是眼镜片中的混浊物，导致全世界50％的失明。进行本研究以评估亚硒酸钠诱导的草木瓜种子在体外和体内白内障中的抗白内障潜力。体外摘除晶状体的大鼠晶状体在器官培养物中维持，该器官培养物中仅含杜贝克改良的Eagles培养基（DMEM）或还含100 µM亚硒酸盐，分别作为正常组和对照组。对于测试组，在培养基中补充亚硒酸盐和太子参-草的水提取物。将镜片在37℃下孵育24小时。孵育后，处理镜片以估计还原型谷胱甘肽（GSH），脂质过氧化产物（丙二醛）和抗氧化酶。通过皮下注射亚硒酸钠（25 µmol / kg体重）在9天大的大鼠中诱发体内亚硒酸钠白内障。在亚硒酸盐激发前4小时，给试验组的动物注射不同剂量的红叶茴香水提取物。与正常晶状体相比，在对照中观察到GSH下降和丙二醛水平上升。猪腥草显着（P <0.01）恢复了谷胱甘肽和降低了丙二醛水平。观察到超氧化物歧化酶（P <0.01），过氧化氢酶（P <0.01），谷胱甘肽过氧化物酶（P <0.01）和谷胱甘肽S-转移酶（P <0.01）等抗氧化酶的活性得到了显着恢复。与对照相比，草丹参补充组。在体内，在治疗组中没有发现眼睛患有核性白内障，而在对照组中则为72.5％。茴香草具有抗氧化特性，可防止白内障。值得进一步研究以探讨其在人白内障中的作用。

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