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首页> 外文期刊>Biology Bulletin >Electron Microscopy Description of Cardiomyocytes from the Left Ventricle of Rat Heart after Apoptosis Induction by Isoproterenol
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Electron Microscopy Description of Cardiomyocytes from the Left Ventricle of Rat Heart after Apoptosis Induction by Isoproterenol

机译:异丙肾上腺素诱导凋亡后大鼠心脏左心室心肌细胞的电镜观察

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Changes in cardiomyocytes from the left ventricle of rat heart were studied by light and electron microscopic and morphometric methods in the myocardial regions neighboring necrotic foci formed after the injection of 80 mg/kg β adrenomimetic isoproterenol. TUNEL assay was used to detect apoptotic cardiomyocytes. Three types of cardiomyocytes (A, B, and C) differing by the ultrastructure of the nucleus and the degree of mitochondrial changes were identified at all studied stages of necrotic focus development (4-48 h). B and C type cardiomyocytes could represent cells at different stages of apoptosis. The apoptotic changes in cardiomyocytes proved to prevail in early lesion foci (4-18 h), while cardiomyocytes at later stages were prone to necrosis; cardiomyocytes can exhibit signs of apoptosis and necrosis at the same time.
机译:通过光,电镜和形态学方法研究了大鼠在注射80 mg / kgβ肾上腺素异丙肾上腺素后形成的坏死灶附近的心肌区域中大鼠左心室心肌细胞的变化。 TUNEL法用于检测凋亡的心肌细胞。在坏死灶发展的所有研究阶段(4-48小时),鉴定出三种类型的心肌细胞(A,B和C),它们的原子核超微结构和线粒体变化程度不同。 B型和C型心肌细胞可以代表处于凋亡不同阶段的细胞。心肌细胞的凋亡变化在病变早期(4-18 h)占优势,后期的心肌细胞容易坏死。心肌细胞可同时显示凋亡和坏死的迹象。

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