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首页> 外文期刊>Biology Bulletin >Somatic Embryogenesis and Morphogenetic Potential of Spring Barley (Hordeum vulgare L.) in the System of Technological Genetic Transformation
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Somatic Embryogenesis and Morphogenetic Potential of Spring Barley (Hordeum vulgare L.) in the System of Technological Genetic Transformation

机译：技术遗传转化体系中大麦的体细胞胚发生和形态发生潜能

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摘要

We studied somatic embryogenesis and morphogenetic potential in young embryos of 17 spring barley cultivars. A considerable effect of the genotype as well as of certain biologically active compounds in the growth medium on the frequency of embryogenic callus formation and regenerative capacity was observed. The Murashige and Skoog medium complemented with myoinositol and casein hydrolysate is suitable for embryogenic callus induction. The regeneration medium should be complemented by hormones, 2.5 mg/l auxin 2,4-D and 0.1-0.5 mg/l cytokinin 6-BAP, to increase the frequency of somatic embryo maturation. The presence of cefotaxime and high level of copper are desirable to increase the efficiency of callogenesis, regeneration, and callus quality. Three cultivars with economically valuable characters and high morphogenetic potential were recommended for development of efficient technology for barley transformation.
机译：我们研究了17个春季大麦品种年轻胚中的体细胞胚发生和形态发生潜能。观察到基因型以及生长培养基中某些生物活性化合物对胚胎发生愈伤组织形成频率和再生能力的显着影响。 Murashige和Skoog培养基补充有肌醇和酪蛋白水解产物，适用于诱导胚性愈伤组织。再生培养基应补充激素，2.5 mg / l生长素2,4-D和0.1-0.5 mg / l细胞分裂素6-BAP，以增加体细胞胚成熟的频率。需要头孢噻肟和高含量的铜以提高愈伤组织发生，再生和愈伤组织质量的效率。建议开发具有经济价值和高形态发生潜力的三个品种，以发展大麦转化的高效技术。

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