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首页> 外文期刊>Biology Bulletin >Adaptive Alterations in the Ultrastructure of Chloroplasts and the Contents of Pigments and Sugars under Low Temperature Hardening of Potato Plants: Role of Δ12 Acyl-Lipid Desaturase
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Adaptive Alterations in the Ultrastructure of Chloroplasts and the Contents of Pigments and Sugars under Low Temperature Hardening of Potato Plants: Role of Δ12 Acyl-Lipid Desaturase

机译:马铃薯植物低温硬化过程中叶绿体超微结构及色素和糖含量的适应性变化:Δ12酰基脂质去饱和酶的作用

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摘要

Alterations in the ultrastructure of chloroplasts and the content of sugars during low-temperature hardening (5 degrees C, 6 days) of potato plants (Solanum tuberosum L., cv. Desnitsa) and a transformed line with the desA gene of 12 acyl-lipid desaturase Synechocystis sp. RCC 6803 were studied. It was noted that during hardening, sugars were accumulated in the leaves of both lines, but with greater speed in the leaves of the transformants. In control plants after hardening, an increase in the total number of thylakoid membranes and in the number of plastoglobules per chloroplast were obtained accompanied by a decrease in the chloroplast cross-sectional area and starch grain area. It was found that chloroplasts of transformed plants, which were different from the control plants in the higher absolute content of membrane lipids and the amount of polyunsaturated fatty acids, corresponded to the hardened plants before cold exposure by some ultrastructural parameters. In the early period of low-temperature hardening, the transformed plants were more adaptable, according to the data on the sugar content.
机译:马铃薯植株(Solanum tuberosum L.,cv。Desnitsa)的低温硬化(5摄氏度,6天)和含有12个酰基脂质的desA基因的转化株期间叶绿体超微结构的变化和糖含量去饱和酶集胞藻研究了RCC 6803。注意到在硬化期间,糖在两条品系的叶子中积累,但是在转化体的叶子中以更快的速度积累。在硬化后的对照植物中,获得了类囊体膜的总数和每个叶绿体的质体球数的增加,同时叶绿体横截面积和淀粉颗粒面积的减少。发现转化植物的叶绿体与对照植物的不同之处在于,膜脂质的绝对含量较高和多不饱和脂肪酸的量较高,在某些超微结构参数的作用下,它们与冷暴露前的硬化植物相对应。根据糖含量的数据,在低温硬化的早期,转化的植物更具适应性。

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