Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M. rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))

Zhigarev I. A.; Zhigarev D. I.; Alpatov V. V.; Lapkovsky V. V.; Malygin V. M.; Simak S. V.

首页> 外文期刊>Biology Bulletin >Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M. rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))

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Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M. rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))

机译：蛋白聚丙烯酰胺凝胶电泳作为常见的田鼠兄弟种鉴定方法（田鼠（Microtus arvalis Pallas，1779年）和罗氏沼虾（M. rossiaemeridionalis Ognev，1924年）（Rodentia，Cricetidae）

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A new protocol for the identification of two sibling species of voles Microtus arvalis and M. rossiaemeridionalis was proposed by electrophoresis of blood proteins in polyacrylamide gel (PAGE). Animals captured in ten districts of Moscow, Kaluga, and Samara oblasts and Moscow natural territories (1030 individuals), as well as those taken from vivarium collections (five hybrids of the two species), were studied. A comparison of electrophoresis in PAGE, agarose gel, and cellulose acetate plates was carried out. The use of different organs and tissues for species identification was assessed. The relative electrophoretic mobility and the mass of a species-specific blood protein of the southern vole were determined.

机译：通过电泳聚丙烯酰胺凝胶（PAGE）中的血液蛋白，提出了一种鉴定田鼠田鼠和野蔷薇的两个同胞物种的新方案。研究了在莫斯科，卡卢加州和萨马拉州和莫斯科自然地区十个地区捕获的动物（1030只），以及从动物饲养场采集的动物（两种动物的五个杂种）。在PAGE，琼脂糖凝胶和醋酸纤维素板上进行电泳比较。评估了不同器官和组织用于物种鉴定的用途。确定了南部田鼠的相对电泳迁移率和特定物种血液蛋白的质量。

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《Biology Bulletin》 |2019年第4期|353-360|共8页
作者
Zhigarev I. A.; Zhigarev D. I.; Alpatov V. V.; Lapkovsky V. V.; Malygin V. M.; Simak S. V.;
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Russian Acad f Sci Severtsov Inst Ecol & Evolut Moscow 119071 Russia|Moscow State Pedag Univ Inst Biol & Chem Moscow 129164 Russia;

Russian Acad f Sci Severtsov Inst Ecol & Evolut Moscow 119071 Russia|Pirogov Russian Natl Res Med Univ Moscow 117997 Russia;

Moscow State Pedag Univ Inst Biol & Chem Moscow 129164 Russia;

Moscow MV Lomonosov State Univ Fac Biol Moscow 119234 Russia;

Nayanova Samara State Reg Acad Samara 443001 Russia;

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Protein Polyacrylamide Gel Electrophoresis as a Common Vole Sibling Species Identification Method (Microtus arvalis Pallas, 1779 and M. rossiaemeridionalis Ognev, 1924 (Rodentia, Cricetidae))

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