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Cryopreservation of Drosophila or How to Save on Collection Maintenance

机译:冷冻保存果蝇或如何节省收集维护

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摘要

An analysis of the literature on the cryopreservation of Drosophila melanogaster, the collections of which include hundreds of thousands of lines, was conducted. It was noted that the cryopreservation of D. melanogaster embryos is possible only at the 14th stage of development after permeabilization of the chorion, which prevents the penetration of cryoprotectants, and succeeds in 3% of cases. The successful cryopreservation of Drosophila larvae and imago was carried out so far only for the Chymomyza costata species. It was established that it is possible to save the cost of maintaining the collection of D. melanogaster by extending ontogenesis due to reducing the feed calorie content and the temperature, as well as due to shortening of the light period.
机译:对果蝇黑素糖蛋白酶冷冻保存的文献分析,其收集包括数十万条线。有人指出,D. melanogaster胚胎的冷冻保存仅在绒毛膜渗透后的第14阶段是可能的,这可以防止冷冻保护剂的渗透,并在3%的情况下成功。到目前为止,仅对Chymomyza Costata物种进行了成功的果蝇和Imago的冷冻保存。已经确定,通过降低饲料卡路里含量和温度,以及由于光周期的缩短,可以通过扩展血管生成来节省维持D. melanogaster的收集的成本。

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  • 来源
    《Biology Bulletin》 |2019年第6期|544-546|共3页
  • 作者

    Zakharenko L. P.;

  • 作者单位

    Russian Acad Sci Inst Cytol & Genet Siberian Branch Novosibirsk 630090 Russia|Novosibirsk State Univ Novosibirsk 630090 Russia;

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  • 正文语种 eng
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