Use of Plant Antimicrobial Peptides in in vitro Embryogenic Cultures of Larix sibirica

Tretyakova I. N.; Rogozhin E. A.; Pak M. E.; Petukhova I. A.; Shuklina A. S.; Pahomova A. P.; Sadykova V. S.

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Use of Plant Antimicrobial Peptides in in vitro Embryogenic Cultures of Larix sibirica

机译：植物抗菌肽在Larix Sibirica体外胚胎培养中的使用

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The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated fromAmarantus retroflexus(seeds),Nigella sativa(seeds), andElytrigia elongata(spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

机译：研究了植物抗微生物肽对愈伤组织和胚胎悬浮体的开始，形成细胞胚胎的形成，以及西伯利亚落叶松的再生剂的形成。蛋白质/肽提取物来自来自retroflexus（种子），nigella sativa（种子），andelytrigia elongata（spikelets）被用作植物来源的物体。在引发胚胎培养物和体细胞胚发芽的开始下已经引入营养培养基中的肽。发现了肽对西伯利亚落叶松胚胎培养形成的刺激作用。没有观察到对照和实验再生体的生长动态的其他差异。本研究应该有助于提高西伯利亚落叶松的克隆种类植物的免疫力。

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《Biology Bulletin》 |2020年第3期|225-236|共12页
作者
Tretyakova I. N.; Rogozhin E. A.; Pak M. E.; Petukhova I. A.; Shuklina A. S.; Pahomova A. P.; Sadykova V. S.;
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Russian Acad Sci Sukachev Inst Forests Siberian Branch Div Fed Res Ctr Krasnoyarsk Sci Ctr Krasnoyarsk 660036 Russia|Siberian Fed Univ Krasnoyarsk 660041 Russia;

Russian Acad Sci Shemyakin Ovchinnikov Inst Bioorgan Chem Moscow 117997 Russia|Gause Inst New Antibiot Moscow 119021 Russia|Tyumen State Univ Tyumen 625003 Russia;

Russian Acad Sci Sukachev Inst Forests Siberian Branch Div Fed Res Ctr Krasnoyarsk Sci Ctr Krasnoyarsk 660036 Russia;

Siberian Fed Univ Krasnoyarsk 660041 Russia;

Russian Acad Sci Sukachev Inst Forests Siberian Branch Div Fed Res Ctr Krasnoyarsk Sci Ctr Krasnoyarsk 660036 Russia;

Russian Acad Sci Sukachev Inst Forests Siberian Branch Div Fed Res Ctr Krasnoyarsk Sci Ctr Krasnoyarsk 660036 Russia;

Gause Inst New Antibiot Moscow 119021 Russia;

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1. Use of Antimicrobial Peptides Secreted by Trichoderma Micromycetes to Stimulate Embryogenic Cultures of Larix sibirica [J] . Tretyakova I. N., Park M. E., Baranova A. A., Russian Journal of Developmental Biology . 2018,第6期

机译：使用Trichoderma微核分泌的抗微生物肽刺激Larix Sibirica的胚性培养物
2. Somatic Polyembriogenesis of Larix sibirica in Embryogenic in vitro Culture [J] . Tretyakova I. N., Park M. E. Russian Journal of Developmental Biology . 2018,第4期

机译：雄性植物在胚胎外培养中的体细胞多重细胞发生
3. Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica [J] . Goryachkina Olga V., Park Maria E., Tretyakova Iraida N., Cytologia : International journal of cytology . 2018,第3期

机译：Larix Sibirica的青少年和长期胚胎培养的细胞遗传学稳定性
4. The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture [C] . Ingus Skadins, Juta Kroica, llze Salma International Baltic Conference of Engineering Materials and Tribology . 2017

机译：复合材料植入和细菌培养后炎症细胞因子和抗微生物肽的水平
5. Analysis of Mutator activity in embryogenic callus cultures and regenerated plants of Zea mays L. [D] . James, Martha Graham. 1989

机译：玉米胚性愈伤组织培养和再生植物中突变体活性的分析
6. In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora [O] . Rafael J. Mendes, Laura Regalado, João P. Luz, 2021

机译：对植物病原体Erwinia Amylovora的五种抗菌肽的体外评价
7. Cytogenetic peculiarities of Larix sibirica Ledeb. embryogenic cell lines in in vitro culture [O] . Olga V. Goryachkina, Maria E. Park, Iraida N. Tretyakova 2017

机译：Larix Sibirica LadeB的细胞遗传学特性。体外培养中的胚胎细胞系
8. Direct seeding of Sitka spruce (Picea sitchensis (Bong.) Carr.), lodgepole pine (Pinus Contorta Dougl. v. contorta) and Siberian larch (Larix sibirica Ledeb.), on scarified seed spots in southern Iceland, using various methods [R] . Petursson, J. G. 1995

机译：使用各种方法直接播种sitka云杉（picea sitchensis（Bong。）Carr。），lodgepole pine（pinus Contorta Dougl。v.controrta）和西伯利亚落叶松（Larix sibirica Ledeb。），在冰岛南部的松散种子斑点上播种

Use of Plant Antimicrobial Peptides in in vitro Embryogenic Cultures of Larix sibirica

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