Community structure of the microbiota in the floodwater of a Japanese paddy field estimated by restriction fragment length polymorphism and denaturing gradient gel electrophoresis pattern analyses

Makoto Kimura; Taketoshi Shibagaki; Yasunori Nakajima; Kazuo Matsuya; Makoto Ikenaga

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Community structure of the microbiota in the floodwater of a Japanese paddy field estimated by restriction fragment length polymorphism and denaturing gradient gel electrophoresis pattern analyses

机译：利用限制性片段长度多态性和变性梯度凝胶电泳图谱分析估算日本稻田洪水区微生物群落结构

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摘要

The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-denaturing gradient gel electrophoresis (DGGE) patterns of 16S rDNA were studied to elucidate the effects of the type of fertilization and the growth stage of rice plants on the community structure of the microbiota in the floodwater of a Japanese paddy field under a long-term fertilizer trial. From the mid tillering stage, a higher pH and temperature were observed in the plot without fertilization (NoF plot) than in the plots supplied with chemical fertilizers (CF plot) and with compost (CM plot). DNA fragments specific to the respective plots and common to every plot were detected after the digestion of PCR products by restriction enzymes. Cluster analysis separated the RFLP and DGGE patterns of the microbiota in the floodwater into four clusters; the microbiota in (1) the NoF plot, (2) the CF plot, (3) the CM plot, and (4) the CF and CM plots in the early growing stage. The effect of fertilizer application on the community structure was more conspicuous than that of seasonal variation.

机译：研究了16S rDNA的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和PCR-变性梯度凝胶电泳（DGGE）模式，以阐明施肥类型和水稻生长阶段对群落结构的影响。长期肥料试验下日本稻田洪水中的微生物群从分till中期开始，未施肥的地块（NoF地块）的pH和温度要高于化学肥料和堆肥（CM地块）的pH和温度。用限制性内切酶消化PCR产物后，检测出各样区特有的DNA片段和每个样区共有的DNA片段。聚类分析将洪水中微生物群的RFLP和DGGE模式分为四个聚类。（1）NoF图，（2）CF图，（3）CM图和（4）生长初期的CF和CM图中的微生物群。施肥对群落结构的影响比季节变化更为明显。

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来源
《Biology and Fertility of Soils》 |2002年第4期|306-312|共7页
作者
Makoto Kimura; Taketoshi Shibagaki; Yasunori Nakajima; Kazuo Matsuya; Makoto Ikenaga;
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作者单位

Graduate School of Bioagricultural Sciences Nagoya University Chikusa-ku Nagoya 464-8601 Japan;

Graduate School of Bioagricultural Sciences Nagoya University Chikusa-ku Nagoya 464-8601 Japan;

Laboratory of Crop Science Aichi-ken Anjo Research and Extension Centre Anjo 446-0073 Japan;

Laboratory of Crop Science Aichi-ken Anjo Research and Extension Centre Anjo 446-0073 Japan;

Graduate School of Bioagricultural Sciences Nagoya University Chikusa-ku Nagoya 464-8601 Japan;

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正文语种 eng
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关键词
Community structure; Denaturing gradient gel electrophoresis; Floodwater; Paddy field; Restriction fragment length polymorphism;

机译：社区结构;变性梯度凝胶电泳;洪水;稻田;限制性片段长度多态性;

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Community structure of the microbiota in the floodwater of a Japanese paddy field estimated by restriction fragment length polymorphism and denaturing gradient gel electrophoresis pattern analyses

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