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Mutation screening method comprises coupling single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE)
Mutation screening method comprises coupling single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE)
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机译：突变筛选方法包括偶联单链构象多态性（SSCP）和变性梯度凝胶电泳（DGGE）
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摘要
A mutation screening method (I), is new and comprises the coupling of single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) through the use of a nested polymerase chain reaction (PCR) primer with a GC adapter sequence. A mutation screening method (I), is new and comprises the coupling of single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) through the use of a nested polymerase chain reaction (PCR) primer with a GC adapter sequence, using: (1) SSCP analysis of the nested PCR products (with GC adapters) of all samples; (2) DGGE mutation analysis of the nested PCR products (with GC adapters) plus attached universal GC clamps of all samples showing no mutation signal after SSCP analysis; (3) sequence analysis of the mutations detected by one of the two screening methods; and optionally (4) post-amplification using a universal biotin-labeled sequence primer.
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