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首页> 外文期刊>Biomass & bioenergy >Mechanism of carbon partitioning towards starch and triacylglycerol in Chlorella vulgaris under nitrogen stress through whole-transcriptome analysis
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Mechanism of carbon partitioning towards starch and triacylglycerol in Chlorella vulgaris under nitrogen stress through whole-transcriptome analysis

机译：通过整体转录组分析在氮气胁迫下Navolla Ventgaris的碳和三酰基甘油的机制

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摘要

This study aims to elucidate the mechanism of carbon partitioning towards starch and triacylglycerol (TAG) under nitrogen stress. Whole-transcriptome analysis was performed using RNA sequencing before being validated with qRT-PCR. The strain, Chlorella vulgaris UPSI-JRM01, exhibited a two-stage response to nitrogen stress: ⅰ) carbohydrate accumulation, plastid protein degradation and amino acid biosynthesis; and ⅱ) lipid accumulation, carbohydrate degradation and DNA damage. In the first stage, the genes responsible for plastid protein degradation (CD4A), amino acid biosynthesis (arg, GLSN, AHCY, win1), carbon fixation (RuBisCO, GapC, fba, TK) and starch synthesis (SS, SBE2) were upregulated. In the second stage, the upregulation of genes responsible for starch degradation (SP), glycolysis (PFO, PK, ACS), fatty acid biosynthesis (FabH, accD) and TAG accumulation (DGAT2) were observed. By contrast, the components of photosystem Ⅰ (PsbD, PsbC, PsbB), photosystem Ⅱ (PsaA), cytochrome b6/f (PetA, PetG) and F-type ATPase (beta, alpha) were downregulated. The results suggested that nitrogen stress triggered high starch accumulation before the carbon was partitioned towards triacylglycerol (TAG) for long-term energy storage by two different pathways: chloroplastic TAG synthesis and glycerolipid metabolism. The starch build-up functions served as a rapid response to nitrogen stress, whereas subsequent lipid accumulation benefited the long-term storage of energy. This research provides in-depth understanding of the metabolic changes triggered by nitrogen stress.
机译：该研究旨在在氮气胁迫下阐明碳分配碳分配的机理和三酰基甘油（标签）。在用QRT-PCR验证之前使用RNA测序进行全转录组分析。 Chlarla Valugaris Upsi-JRM01菌株表现出对氮胁迫的两级反应：Ⅰ）碳水化合物积累，体液蛋白质降解和氨基酸生物合成; Ⅱ）脂质积累，碳水化合物降解和DNA损伤。在第一阶段，上调负责体积蛋白质降解（CD4a），氨基酸生物合成（Arg，Glsn，Ahcy，Win1），碳固定（Rubisco，GaPC，FBA，TK）和淀粉合成（SS，SS，SS，SS，SS，SS，SS，SS2）中的基因。在第二阶段，观察到负责淀粉降解（SP），糖酵解（PFO，PK，AC），脂肪酸生物合成（FabH，ACCD）和标签积累（DGAT2）的基因的上调。相比之下，下调照相Ⅰ（PSBD，PSBC，PSBB），光系统Ⅱ（PSAA），细胞色素B6 / F（PETA，PETG）和F型ATP酶（β，α）的组分。结果表明，在碳被朝向三酰基甘油（标签）划分为长期能量储存之前，氮胁迫触发高淀粉积累：叶片标签合成和甘油脂代谢。淀粉积聚功能作为对氮胁迫的快速响应，而随后的脂质积累受益于能量的长期储存。本研究提供了对由氮胁迫引发的代谢变化的深入了解。

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  • 来源
    《Biomass & bioenergy》 |2020年第7期|105600.1-105600.14|共14页
  • 作者

    Norazela Nordin; Norjan Yusof; Toshinari Maeda; Nurul Asyifah Mustapha; Mohd Zulkhairi Mohd Yusoff; Raja Farhana Raja Khairuddin;

  • 作者单位

    Department of Biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris 35900 Tanjong Malim Perak Darul Ridzuan Malaysia;

    Department of Biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris 35900 Tanjong Malim Perak Darul Ridzuan Malaysia;

    Department of Biological Functions and Engineering Graduate School of Life Science and Systems Engineering Kyushu Institute of Technology 2-4 Hibikino Wakamatsu-ku Kitakyushu Fukuoka 808-0196 Japan;

    Department of Biological Functions and Engineering Graduate School of Life Science and Systems Engineering Kyushu Institute of Technology 2-4 Hibikino Wakamatsu-ku Kitakyushu Fukuoka 808-0196 Japan;

    Department of Bioprocess Technology Faculty of Biotechnology and Biomolecular Sciences Universiti Putra Malaysia 43400 Serdang Selangor Malaysia;

    Department of Biology Faculty of Science and Mathematics Universiti Pendidikan Sultan Idris 35900 Tanjong Malim Perak Darul Ridzuan Malaysia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Chlorella vulgaris; Gene expression; Nitrogen stress; RNA sequencing; Carbon partitioning mechanism;

    机译：小球藻vulgaris;基因表达;氮胁迫;RNA测序;碳分区机制;

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