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首页> 外文期刊>Biomass & bioenergy >Enzymatic in situ saccharification of lignocellulosic biomass in ionic liquids using an ionic liquid-tolerant cellulases
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Enzymatic in situ saccharification of lignocellulosic biomass in ionic liquids using an ionic liquid-tolerant cellulases

机译：使用耐离子液体的纤维素酶对木质纤维素生物质在离子液体中的酶促原位糖化

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摘要

Ionic liquids (ILs) have been considered as a class of promising solvents that can dissolve lignocellulosic biomass and then provide enzymatic hydrolyzable holocellulose. However, most of available cellulases are completely or partially inactivated in even low concentrations of ILs. To more fully exploit the benefits of ILs to lignocellulose biorefinery, it is critical to improve the compatibility between cellulase and ILs. In this study, an IL-tolerant cellulase cocktail derived from Penicillium oxalicum GS was isolated from chemicals polluted microhabitats. High hydrolytic activity of the cellulase cocktail was detected in various ILs. In particular, the cellulase cocktail retained 91% initial activity in IL dimethylimidazolium methylphosphonate ([Dmim][(OCH_3)_2PO_2]) (20%, w/v), which was effective in disrupting compact structures of rice straw. Furthermore, the cellulase cocktail even preserved 61% initial activity in 25% (w/ v) [Dmim][(OCH_3)_2PO_2] media. A compatible cellulase-IL system, which combines IL pretreatment and subsequent enzymatic saccharification of lignocellulosic biomass into a single vessel, was then developed based on the cellulase cocktail and [Dmim][(OCH_3)_2PO_2]. Its use significantly improved the saccharification rate of rice straw from 47% to 83% versus the control.
机译：离子液体（ILs）被认为是一类有前途的溶剂，可以溶解木质纤维素生物质，然后提供可酶水解的全纤维素。但是，即使在低浓度的ILs中，大多数可用的纤维素酶也会完全或部分失活。为了更充分地利用白介素对木质纤维素生物精炼厂的好处，提高纤维素酶与白介素之间的相容性至关重要。在这项研究中，从草木青霉GS衍生的耐IL的纤维素酶混合物是从化学污染的微生境中分离出来的。在各种IL中检测到纤维素酶混合物的高水解活性。特别地，纤维素酶混合物在IL二甲基咪唑鎓甲基膦酸酯（[Dmim] [（OCH_3）_2PO_2]）（20％，w / v）中保留了91％的初始活性，这有效地破坏了稻草的紧凑结构。此外，纤维素酶混合物甚至在25％（w / v）[Dmim] [（OCH_3）_2PO_2]培养基中保留了61％的初始活性。然后，基于纤维素酶混合物和[Dmim] [（OCH_3）_2PO_2]，开发了一种兼容的纤维素酶-IL系统，该系统将IL预处理和随后的木质纤维素生物质酶促糖化结合到一个容器中。与对照相比，它的使用显着提高了稻草的糖化率从47％提高到83％。

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  • 来源
    《Biomass & bioenergy》 |2016年第10期|180-186|共7页
  • 作者

    Jiaxing Xu; Xinfeng Wang; Xiaoyan Liu; Jun Xia; Tong Zhang; Peng Xiong;

  • 作者单位

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China;

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China,Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian, 223300, China;

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China;

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China;

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China;

    Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian, 223300, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biomass; Ionic liquid; Pretreatment; Cellulase; Tolerance; Saccharification;

    机译：生物质离子液体;预处理;纤维素酶公差;糖化;

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