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首页> 外文期刊>IEEE Transactions on Biomedical Engineering >Applying Combined Optical Tweezers and Fluorescence Microscopy Technologies to Manipulate Cell Adhesions for Cell-to-Cell Interaction Study
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Applying Combined Optical Tweezers and Fluorescence Microscopy Technologies to Manipulate Cell Adhesions for Cell-to-Cell Interaction Study

机译：应用组合的光镊和荧光显微镜技术来操纵细胞粘附，进行细胞间相互作用研究

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摘要

Cell-to-cell interactions are important for the regulation of various cell activities, such as proliferation, differentiation, and apoptosis. This paper presents an approach to studying cell-to-cell interactions at a single-cell level through manipulating cell adhesions with optical tweezers. Experiments are performed on leukemia cancer cells and stromal cells to demonstrate the feasibility of this method. After the adhesion properties of leukemia cells on stromal cells are characterized, fluorescence intensity is used as a label to study the Wnt signaling pathway of leukemia cells. The activities of the Wnt signaling pathway of K562 cells on M210B4 and HS5 cells are examined based on fluorescence analysis. The reliability of the fluorescence imaging is confirmed through comparison with traditional flow cytometry analysis. The proposed approach will offer new avenues to investigate otherwise inaccessible mechanisms in cell-to-cell interactions.
机译：细胞间相互作用对于调节各种细胞活性（例如增殖，分化和凋亡）非常重要。本文提出了一种通过使用光镊操纵细胞粘附来研究单细胞水平上细胞间相互作用的方法。对白血病癌细胞和基质细胞进行了实验以证明该方法的可行性。表征白血病细胞在基质细胞上的粘附特性后，将荧光强度用作研究白血病细胞Wnt信号通路的标记。基于荧光分析，检查了K562细胞在M210B4和HS5细胞上的Wnt信号通路的活性。通过与传统的流式细胞仪分析比较，可以确认荧光成像的可靠性。所提出的方法将提供新的途径来研究细胞间相互作用中否则无法接近的机制。

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