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首页> 外文期刊>Biomedical Microdevices >Sample preparation: the weak link in microfluidics-based biodetection
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Sample preparation: the weak link in microfluidics-based biodetection

机译：样品制备：基于微流控的生物检测中的薄弱环节

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摘要

As a broad generalization, clinicians and laboratory personnel who use microfluidics-based automated or semi-automated instrumentation to perform biomedical assays on real-world samples are more pleased with the state of the assays than they are with the state of the front-end sample preparation. The end-to-end procedure requires one to collect, manipulate, prepare, and analyze the sample. The appeal of microfluidics for this procedure is partly based on its combination of small size and its ability to process very small liquid volumes, thus minimizing the use of possibly expensive reagents. However, real-world samples are often large and incompatible with the input port and the μm-scale channels of a microfluidic device, and very small liquid volumes can be inappropriate in analyzing low concentrations of target analytes. It can be a worthy challenge to take a raw sample, introduce it into a microfluidics-based system, and perform the sample preparation, which may include separation and concentration of the target analytes, so that one can benefit from the reagent-conserving small volumes and obtain the correct answer when finally implementing the assay of interest.
机译：作为广义的概括，使用基于微流体的自动化或半自动化仪器对真实样品进行生物医学测定的临床医生和实验室人员对测定的状态比对前端样品的状态更满意。制备。端到端程序需要一个程序来收集，操作，准备和分析样品。微流体技术对该程序的吸引力部分是基于其小尺寸的组合以及其处理非常小的液体体积的能力，从而将可能昂贵的试剂的使用减至最少。但是，现实世界中的样品通常很大，并且与微流体设备的输入端口和微米级通道不兼容，非常小的液体体积可能不适用于分析低浓度的目标分析物。采集原始样品，将其引入基于微流体的系统，然后进行样品制备（可能包括目标分析物的分离和浓缩），可能是一个巨大的挑战，这样一来，就可以从少量保存试剂的方法中受益并在最终实施目标分析时获得正确答案。

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