• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomedical Microdevices >Ejection of cell laden RPMI-1640 culture medium by Electrohydrodynamic method
【24h】

Ejection of cell laden RPMI-1640 culture medium by Electrohydrodynamic method

机译：电动流体力学方法喷射细胞载脂蛋白RPMI-1640培养基

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sample deposition based on micro-droplet ejection has broad application prospects in the field of biomedicine. Ejection of RPMI-1640 medium (with and without cells) is investigated experimentally using a home-build electrohydrodynamic (EHD) ejection system, consisting of a liquid supplier and a nozzle, a high voltage source, a droplet collector, and a high speed photography module. High electric voltage is applied between the nozzle and the droplet collector. The liquid surface is electrically charged and the ejection takes place when electric force overcomes the surface tension. The ejection process is studied by using high speed photography and image processing. At low voltage, a stable ejection state is established with ejection frequency ranging from a few to a few tens of Hertz. At high voltage, another stable ejection state is reached with ejection frequency as high as 1300Hz. At the transition voltage range, the ejection exhibits a periodic behaviour. During each cycle, the meniscus rapidly oscillates with gradually increased amplitude, and with several non-uniform droplets ejected at the final stage of the cycle. Human peripheral blood mononuclear cells, after ejection, shows survival rates higher than 79%, manifesting EHD ejection as a promising technique for cell printing.
机译：基于微滴喷射的样品沉积在生物医学领域具有广阔的应用前景。使用自制的电动液力（EHD）喷射系统对RPMI-1640介质（有或没有细胞）的喷射进行了实验研究，该系统由液体供应器和喷嘴，高压源，液滴收集器和高速摄影组成模块。在喷嘴和液滴收集器之间施加高电压。当电力克服表面张力时，液体表面带电并且发生喷射。通过使用高速摄影和图像处理来研究喷射过程。在低电压下，建立稳定的喷射状态，其喷射频率范围为几赫兹到几十赫兹。在高电压下，以高达1300Hz的喷射频率达到另一个稳定的喷射状态。在过渡电压范围内，喷射表现出周期性的行为。在每个周期中，弯月面均以逐渐增大的幅度快速振荡，并在周期的最后阶段喷射出一些不均匀的液滴。射血后，人外周血单核细胞的存活率高于79％，这表明EHD射血是一种有希望的细胞打印技术。

著录项

  • 来源
    《Biomedical Microdevices》 |2019年第3期|64.1-64.9|共9页
  • 作者

    Zhang Haiyi; Wang Can; Jia Ruiwen; Wang Fei; Wang Yiwei; Wang Zhihai; Chen Xi; Wang Xiaolin; Gui Jingang;

  • 作者单位

    Beijing Univ Technol, Fac Informat Technol, Beijing 100124, Peoples R China|Beijing Univ Technol, Key Lab Optoelect Technol, Minist Educ, Beijing 100124, Peoples R China;

    Beijing Univ Technol, Laser Engn Res Inst, Beijing 100124, Peoples R China;

    Beijing Univ Technol, Fac Informat Technol, Beijing 100124, Peoples R China;

    Beijing Univ Technol, Fac Informat Technol, Beijing 100124, Peoples R China;

    Beijing Univ Technol, Fac Informat Technol, Beijing 100124, Peoples R China|Beijing Univ Technol, Key Lab Optoelect Technol, Minist Educ, Beijing 100124, Peoples R China;

    Beijing Univ Technol, Fac Informat Technol, Beijing 100124, Peoples R China|Beijing Univ Technol, Key Lab Optoelect Technol, Minist Educ, Beijing 100124, Peoples R China;

    Capital Med Univ, Beijing Inst Pediat, Beijing Childrens Hosp, Beijing 100035, Peoples R China;

    Capital Med Univ, Beijing Inst Pediat, Beijing Childrens Hosp, Beijing 100035, Peoples R China;

    Capital Med Univ, Beijing Inst Pediat, Beijing Childrens Hosp, Beijing 100035, Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Electrohydrodynamic; Ejection frequency; High speed camera; Cell printing;

    机译：电流性动力学;喷射频率;高速相机;细胞印刷;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号