摘要:目的:通过静电纺丝法制备不同比例PLGA/β-TCP纳米纤维支架,筛选出最合适比例,以便为进一步的体内植入提供依据.方法:利用静电纺丝法制备比例为10:0、9:1、8:2、7:3、6:4、5:5的PLGA/β-TCP纳米纤维支架,扫描电镜观察纤维支架的多孔结构,液体置换法测量支架的孔隙率:分别于1%胰酶PBS溶液中进行体外降解,测定材料的降解性;接触角仪测量材料的接触角,评价其亲水性能.结果:电镜观察显示制备的不同比例的PLGA/β-TCP纤维中(除6:4、5:5组),直径均一,呈相互联通的三维多孔结构,各组支架材料的孔隙率均>80%,其中6:4、5:5组的孔隙率>85%,电镜下观察纤维成分有限,大部分为颗粒状,不具有多孔支架的三维结构;体外降解实验中7:3、6:4、5:5组均在第7周时完全降解.结论:通过静电纺丝法制备不同比例的PLGA/TCP支架材料,9:1、8:2、7:3组均符合骨组织工程要求,体外降解实验中,7:3组的降解率较另两组材料为优,具有用作体内骨修复材料的潜力.%Objective:To prepare the different ratio of PLGA / β-tricalcium phosphate electrospun nanofibrous scaf-folds and select the more suitable ratio so as to procide a basis for further research of implant. Method: The nanofiber scaf-folds of PLGA / β-TCP with different component ratio(10:0,9:1,8:2,7:3,6:4,5:5) were prefabricated by electrospinning technology. The porous structure of nanofiber was observed under scanning electron microscope (SEM), and the porosity was assayed with stereometry .The materials were placed into 1 % pancreatin PBS, then degraded in vitro, measured the biodegradability of the materials. The hydrophilic of the materials was evaluated by the contact angle which measured by contact angle mater. Result: The SEM images showed that the different ratio of PLGA / p-TCP nanofibers (except 6:4 and 5:5) are smooth and uniform with the interwoven and porous morphology.The average porosity of the materials was over 80 % and the porosity of the materials (6:4 and 5:5) was over 85 %.But by SEM the fiber component of this two groups were so limited ,most of them were granular and don not have the three-dimensional structure of porous scaffold :In the duration of in vitro degradation.at 7 weeks,the materials degraded completely in the material (7:3,6:4,5:5)groups. Conclusion:The different ratio of PLGA / β-tricalcium phosphate scaffold materials were prepared by electrospinning technology .The groups of 9:1.8:2 and 7:3 meet the demands of bone tissue engineering. In the duration of in vitro degradation, the degradation rate of 7:3 was better than other two groups and hence, it have potential prospect for bone implantation.