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首页> 外文期刊>Bioprocess and Biosystems Engineering >Oxygen-enriched fermentation of sodium gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions
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Oxygen-enriched fermentation of sodium gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions

机译：黑曲霉富氧发酵葡萄糖酸钠及其对细胞内代谢通量分布的影响

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摘要

Different concentrations of oxygen-enriched air were utilized for sodium gluconate (SG) fermentation by Aspergillus niger. The fermentation time shortened from 20 to 15.5 h due to the increase of volumetric oxygen transfer coefficient (K(L)a) and the formation of more dispersed mycelia when inlet oxygen concentration ascended from 21 to 32%. According to metabolic flux analysis, during the growth phase, extracellular glucose for SG synthesis accounted for 79.0 and 85.3% with air and oxygen-enriched air (25%), respectively, whereas the proportions were 89.4 and 93.0% in the stationary phase. Intracellular glucose consumption decreased in oxygen-enriched fermentation, as cell respiration was more high-efficiently performed. Metabolic profiling indicated that most intermediates in TCA cycle and EMP pathway had smaller pool sizes in oxygen-enriched fermentations. Moreover, the main by-product of citric acid dramatically decreased from 1.36 to 0.34 g L-1 in oxygen-enriched fermentation. And the sodium gluconate yield increased from 0.856 to 0.903 mol mol(-1).
机译：黑曲霉利用不同浓度的富氧空气进行葡萄糖酸钠（SG）发酵。发酵时间从20缩短到15.5 h，这是由于入口氧气浓度从21％升至32％时，体积氧气传递系数（K（L）a）的增加以及形成更分散的菌丝体所致。根据代谢通量分析，在生长阶段，用于SG合成的细胞外葡萄糖分别占空气和富氧空气的25％（分别为79.0％和85.3％），而在静止期则分别为89.4％和93.0％。富氧发酵中细胞内葡萄糖消耗减少，因为细胞呼吸更有效地进行。代谢谱分析表明，TCA循环和EMP途径中的大多数中间体在富氧发酵中具有较小的库大小。此外，在富氧发酵中，柠檬酸的主要副产物从1.36 g L-1急剧降低至0.34 g L-1。葡萄糖酸钠的产率从0.856 mol mol（-1）增加到0.903 mol mol（-1）。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第1期|77-86|共10页
  • 作者

    Shen Yuting; Tian Xiwei; Zhao Wei; Hang Haifeng; Chu Ju;

  • 作者单位

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    Shan Dong Fuyang Biol Technol Co Ltd, Dezhou, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Gluconic acid; Aspergillus niger; Oxygen; Metabolic flux distribution;

    机译：葡萄糖酸;黑曲霉;氧气;代谢通量分布;

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