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首页> 外文期刊>Bioprocess and Biosystems Engineering >Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02: enzymatic properties and efficient functions for acetoin/butanediol and l-valine biosynthesis
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Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02: enzymatic properties and efficient functions for acetoin/butanediol and l-valine biosynthesis

机译：地衣芽孢杆菌WX-02中的乙酰乳酸合酶（AlsS）：乙酰乙酸/丁二醇和l-缬氨酸生物合成的酶学性质和有效功能

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摘要

Acetolactate synthase catalyzes two molecules of pyruvates to form alpha-acetolactate, which is further converted to acetoin and 2,3-butanediol. In this study, by heterologous expression in Escherichia coli, the enzymatic properties of acetolactate synthase (AlsS) from Bacillus licheniformis WX-02 were characterized. Its K (m) and k (cat) for pyruvate were 3.96 mM and 514/s, respectively. It has the optimal activity at pH 6.5, 37 A degrees C and was feedback inhibited by l-valine, l-leucine and l-isoleucine. Furthermore, the alsS-deficient strain could not produce acetoin, 2,3-butanediol, and l-valine, while the complementary strain was able to restore these capacities. The alsS overexpressing strain produced higher amounts of acetoin/2,3-butanediol (57.06 g/L) and l-valine (2.68 mM), which were 10.90 and 92.80% higher than those of the control strain, respectively. This is the first report regarding the in-depth understanding of AlsS enzymatic properties and its functions in B. licheniformis, and overexpression of AlsS can effectively improve acetoin/2,3-butanediol and l-valine production in B. licheniformis. We envision that this AlsS can also be applied in the improvement of acetoin/2,3-butanediol and l-valine production in other microbes.
机译：乙酰乳酸合酶催化两个丙酮酸分子形成α-乙酰乳酸，将其进一步转化为乙酰丁酸酯和2,3-丁二醇。在这项研究中，通过在大肠杆菌中的异源表达，表征了地衣芽孢杆菌WX-02的乙酰乳酸合酶（AlsS）的酶学性质。丙酮酸的K（m）和k（cat）分别为3.96 mM和514 / s。它在pH 6.5、37 A摄氏度下具有最佳活性，并被1-缬氨酸，1-亮氨酸和1-异亮氨酸抑制。此外，alsS缺陷型菌株不能产生乙酰丁二酸，2，3-丁二醇和1-缬氨酸，而互补菌株能够恢复这些能力。 alsS过表达菌株产生的乙酰丙酮/ 2,3-丁二醇（57.06 g / L）和l-缬氨酸（2.68 mM）的量分别比对照菌株高10.90％和92.80％。这是关于深入了解地衣芽孢杆菌中AlsS的酶学性质及其功能的第一份报告，而AlsS的过表达可以有效地改善地衣芽孢杆菌中乙醛/ 2,3-丁二醇和1-缬氨酸的产生。我们预想，该AlsS也可用于改善其他微生物中乙酰化/ 2,3-丁二醇和1-缬氨酸的生产。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第1期|87-96|共10页
  • 作者

    Huo Yanli; Zhan Yangyang; Wang Qin; Li Shunyi; Yang Shihui; Nomura Christopher T.; Wang Changjun; Chen Shouwen;

  • 作者单位

    Lanzhou Jiaotong Univ, Sch Chem & Biol Engn, Lanzhou 730070, Gansu, Peoples R China|Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China|SUNY Syracuse, Coll Environm Sci & Forestry, SUNY ESF, Dept Chem, Syracuse, NY 13210 USA;

    Tobacco Res Inst Hubei Prov, Wuhan 430062, Hubei, Peoples R China;

    Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Environm Microbial Technol Ctr Hubei Prov, Wuhan 430062, Hubei, Peoples R China|Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China;

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  • 关键词

    Acetolactate synthase; Baicllus licheniformis; Acetoin/2,3-butanediol; L-valine; Enzymatic properties;

    机译：乙酰乳酸合酶;地衣芽孢杆菌;乙缩醛/ 2,3-丁二醇;L-缬氨酸;酶学性质;

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