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首页> 外文期刊>Bioprocess and Biosystems Engineering >EMS-induced mutation followed by quizalofop-screening increased lipid productivity in Chlorella sp.
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EMS-induced mutation followed by quizalofop-screening increased lipid productivity in Chlorella sp.

机译：EMS诱导的突变，然后进行quizalofop筛选，增加了小球藻的脂质生产力。

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The objective of this study was to enhance biomass and lipid productivity in Chlorella sp. isolate 6-4 by inducing mutagenesis with two growth inhibitors: the herbicide quizalofop-P-ethyl, a known inhibitor of acetyl-CoA carboxylase (ACCase) activity, and chemical mutagen, ethyl methanesulfonate (EMS), at different concentrations and length of times. The induced-mutagenized microalgae were screened on selective medium containing 10-100 mu M quizalofop. The biomass yield, biomass productivity, lipid content, and lipid productivity of mutagenized microalgae were determined. The result showed that 100-200 mM EMS concentrations and 30 min incubation time were the most effective. Biomass yield and biomass productivity of the mutagenized microalgae E50-30-40, E100-60-40, and E100-30-60 were statistically significant higher than those of the wild type. The mutagenized microalgae E100-30-60 showed that the highest biomass yield and biomass productivity were 111 and 110% higher than the wild type, respectively (p 0.01). Lipid content and lipid productivity of the mutagenized microalgae E200-30-40 were 59 and 53% significantly higher than the wild type, respectively. It should be noted that biomass productivity of the mutagenized microalgae E200-30-40 was not significantly different from E100-30-60, meaning that this microalga strain exhibited highest both biomass and lipid productivity. These results indicated that inducing mutagenesis by EMS subsequently screening by herbicide could lead to enhance biomass and lipid accumulation. Therefore, this methodology could be used for improvement microalgae for biofuel production.
机译：这项研究的目的是提高小球藻的生物量和脂质生产力。通过用两种生长抑制剂诱导诱变来分离6-4：除草剂Quizalofop-P-ethyl，一种已知的乙酰辅酶A羧化酶（ACCase）活性抑制剂，以及化学诱变剂，甲烷磺酸乙酯（EMS），在不同的浓度和时间长度下。在含有10-100μMquizalofop的选择性培养基上筛选诱导诱变的微藻。确定了诱变微藻的生物量产量，生物量生产率，脂质含量和脂质生产率。结果显示100-200 mM EMS浓度和30分钟孵育时间是最有效的。诱变的微藻E50-30-40，E100-60-40和E100-30-60的生物量产量和生物量生产力在统计学上显着高于野生型。诱变的微藻E100-30-60的最高生物量产量和生物量生产率分别比野生型高111％和110％（p <0.01）。诱变的微藻E200-30-40的脂质含量和脂质生产率分别比野生型分别高59％和53％。应该注意的是，诱变的微藻E200-30-40的生物质生产力与E100-30-60并无显着差异，这意味着该微藻菌株的生物质和脂质生产力均最高。这些结果表明，通过EMS诱导诱变，随后通过除草剂筛选可以诱使生物量和脂质积累增加。因此，该方法学可用于改进微藻以生产生物燃料。

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