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New lipopeptide produced by Corynebacterium aquaticum from a low- cost substrate

机译：水生棒状杆菌从低成本底物生产的新脂肽

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Conventional biosurfactants have high production costs. Therefore, the use of low-cost carbon sources for their production is attractive for industry. The ability to remain stable under various environmental conditions further extends industrial application. Here we aimed to evaluate the stability of a new lipopeptide produced by Corynebacterium aquaticum using fish residue as an unconventional energy source. The biosurfactant was produced using 3% fish residue, 2% of the microorganism, and mineral medium. Biosurfactant characterization was performed by thin layer chromatography (TLC), as well as by testing its infrared, surface tension, emulsifying activity, and ionic character. The stability of the biosurfactant was evaluated by testing its surface tension at a range of temperatures, pH, and saline concentrations, as well as after 6 months of storage. The biosurfactant was characterized as a lipopeptide due to its retention time, which was coincident with the amino acid and lipid chains obtained in the TLC analysis, being confirmed by some regions of absorption verified in the infrared analysis. The surface tension and emulsifying activity of the biosurfactant were 27.8 mN/m and 87.6%, respectively, and showed anionic character. The biosurfactant was stable at temperatures of 20 to 121 A degrees C, in saline concentrations of 1 to 7%, and at pH close to neutrality. Based on our findings, it is possible to use unconventional sources of energy to produce a lipopeptide biosurfactant that can act under various environments.
机译：常规生物表面活性剂的生产成本高。因此，将低成本碳源用于其生产对工业具有吸引力。在各种环境条件下保持稳定的能力进一步扩展了工业应用。在这里，我们旨在评估使用鱼渣作为非常规能源的水产棒状杆菌产生的新脂肽的稳定性。使用3％的鱼渣，2％的微生物和矿物培养基生产生物表面活性剂。生物表面活性剂的表征通过薄层色谱法（TLC）进行，并通过测试其红外，表面张力，乳化活性和离子特性进行。通过在温度，pH和盐浓度范围内以及储存6个月后测试其表面张力来评估生物表面活性剂的稳定性。由于其保留时间，该生物表面活性剂被表征为脂肽，这与TLC分析中获得的氨基酸和脂质链相吻合，并通过红外分析中证实的某些吸收区域得到证实。该生物表面活性剂的表面张力和乳化活性分别为27.8mN / m和87.6％，并显示出阴离子特性。生物表面活性剂在20至121 A的温度，1至7％的盐水浓度以及接近中性的pH值下稳定。根据我们的发现，有可能使用非常规能源来生产可以在各种环境下起作用的脂肽生物表面活性剂。

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