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首页> 外文期刊>Bioprocess and Biosystems Engineering >Studies on substrate utilisation in L-valine-producing Corynebacterium glutamicum strains deficient in pyruvate dehydrogenase complex
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Studies on substrate utilisation in L-valine-producing Corynebacterium glutamicum strains deficient in pyruvate dehydrogenase complex

机译：丙酮酸脱氢酶复合物缺陷的产L-缬氨酸的谷氨酸棒杆菌菌株底物利用的研究

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The pyruvate dehydrogenase complex was deleted to increase precursor availability in Corynebacterium glutamicum strains overproducing L-valine. The resulting auxotrophy is treated by adding acetate in addition glucose for growth, resulting in the puzzling fact of gluconeogenic growth with strongly reduced glucose uptake in the presence of acetate in the medium. This result was proven by intracellular metabolite analysis and labelling experiments. To increase productivity, the SugR protein involved in negative regulation of the phosphotransferase system, was inactivated, resulting in enhanced consumption of glucose. However, the surplus in substrate uptake was not converted to L-valine; instead, the formation of up to 289 μM xylulose was observed for the first time in C. glutamicum. As an alternative to the genetic engineering solution, a straightforward process engineering approach is proposed. Acetate limitation resulted in a more efficient use of acetate asrncosubstrate, shown by an increased biomass yield and improved L-valine formation.
机译：删除丙酮酸脱氢酶复合物以增加谷氨酸棒杆菌菌株过量生产L-缬氨酸的前体可用性。通过在葡萄糖中添加乙酸盐以促进生长来处理营养缺陷型，导致糖原异生生长令人费解的事实，在培养基中存在乙酸盐的情况下葡萄糖摄取大大降低。细胞内代谢产物分析和标记实验证明了这一结果。为了提高生产率，使磷酸转移酶系统负调控中涉及的SugR蛋白失活，导致葡萄糖消耗增加。然而，底物摄取的剩余并没有转化为L-缬氨酸。相反，首次在谷氨酸棒杆菌中观察到高达289μM的木酮糖的形成。作为基因工程解决方案的替代方法，提出了一种简单的过程工程方法。乙酸盐的限制导致乙酸盐作为底物的更有效利用，表现为增加的生物量产量和改善的L-缬氨酸形成。

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