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首页> 外文期刊>Bioprocess and Biosystems Engineering >Butyrate-based n-butanol production from an engineered Shewanella oneidensis MR-1
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Butyrate-based n-butanol production from an engineered Shewanella oneidensis MR-1

机译：从Shewanella oneidensis MR-1生产基于丁酸酯的正丁醇

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摘要

n-Butanol is considered as the next-generation biofuel, because its physiochemical properties are very similar to fossil fuels and it could be produced by Clostridia under anaerobic culture. Due to the difficulties of strict anaerobic culture, a host which can be used with facultative environment was being searched for n-butanol production. As an alternative, Shewanella oneidensis MR-1, which is known as facultative bacteria, was selected as a host and studied. A plasmid containing adhE2 encoding alcohol dehydrogenase, various CoA transferases (ctfAB, atoAD, pct, and ACT), and acs encoding acetyl-CoA synthetase were introduced and examined to S. oneidensis MR-1 to produce n-butanol. As a result, ctfAB, acs, and adhE2 overexpression in S. oneidensis-pJM102 showed the highest n-butanol production in the presence of 2% of N-acetylglucosamine (NAG), 0.3% of butyrate, and 0.1 mM of IPTG for 96 h under microaerobic condition. When more NAG and butyrate were fed, n-butanol production was enhanced, producing up to 160 mg/L of n-butanol. When metal ions or extra electrons were added to S. oneidensis-pJM102 for n-butanol production, metal ion as electron acceptor or supply of extra electron showed no significant effect on n-butanol production. Overall, we made a newly engineered S. oneidensis that could utilize NAG and butyrate to produce n-butanol. It could be used in further microaerobic condition and electricity supply studies.
机译：正丁醇被认为是下一代生物燃料，因为其理化特性与化石燃料非常相似，并且可由梭菌在厌氧培养条件下生产。由于严格厌氧培养的困难，正在寻找可在兼职环境中使用的宿主以生产正丁醇。作为替代方案，选择了Shewanella oneidensis MR-1（称为兼性细菌）作为宿主并进行了研究。引入含有编码醇脱氢酶adhE2，各种CoA转移酶（ctfAB，atoAD，pct和ACT）和编码乙酰辅酶A合成酶的acs的质粒，并将其检测到拟南芥MR-1中以生产正丁醇。结果，在96％的N-乙酰氨基葡萄糖（NAG），0.3％的丁酸和0.1mM的IPTG存在下，沙丁鱼-pJM102中的ctfAB，acs和adhE2过表达显示出最高的正丁醇产量。 h在有氧条件下。当添加更多的NAG和丁酸酯时，正丁醇的产量会提高，最多可生产160 mg / L的正丁醇。当将金属离子或多余的电子添加到沙门氏菌-pJM102中生产正丁醇时，金属离子作为电子受体或多余电子的供应对正丁醇的生产没有显着影响。总体而言，我们制造了一种新近工程化的沙门氏菌，可以利用NAG和丁酸酯生产正丁醇。它可以用于进一步的有氧条件和电力供应研究。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第8期|1195-1204|共10页
  • 作者

    Jeon Jong-Min; Song Hun-Suk; Lee Doo-Geun; Hong Ju Won; Hong Yoon Gi; Moon Yu-Mi; Bhatia Shashi Kant; Yoon Jeong-Jun; Kim Wooseong; Yang Yung-Hun;

  • 作者单位

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Korea Inst Ind Technol KITECH, Intelligent Sustainable Mat R&D Grp, Chonan Si 31056, Chungcheongnam, South Korea;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

    Korea Inst Ind Technol KITECH, Intelligent Sustainable Mat R&D Grp, Chonan Si 31056, Chungcheongnam, South Korea;

    Brown Univ, Rhode Isl Hosp, Alpert Med Sch, Div Infect Dis, Providence, RI 02903 USA;

    Konkuk Univ, Coll Engn, Dept Biol Engn, Seoul 05029, South Korea;

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  • 正文语种 eng
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  • 关键词

    n-Butanol; Shewanella oneidensis MR-1; Electron transfer;

    机译：正丁醇;沙瓦氏假单胞菌MR-1;电子转移;

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