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首页> 外文期刊>Bioprocess and Biosystems Engineering >Strain selection of microalgae isolated from Tunisian coast: characterization of the lipid profile for potential biodiesel production
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Strain selection of microalgae isolated from Tunisian coast: characterization of the lipid profile for potential biodiesel production

机译：从突尼斯海岸分离的微藻的菌株选择：表征潜在生物柴油生产的脂质谱

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Microalgae could be of importance for future biodiesel production as an alternative for a third generation of biofuels. To select the most appropriate strain for biodiesel production, three microalgae species, namely Isochrysis sp., Nannochloropsis maritima and Tetraselmis sp., isolated from Tunisian coast, were biochemically characterized. Initially, gas chromatography analysis showed that Isochrysis sp. and N. maritima contained 5- and 10-fold total fatty acids, respectively, more than Tetraselmis sp. Then, the two microalgae Isochrysis sp. and N. maritima were subject to random mutagenesis using ultraviolet-C radiation. Subsequently, a total of 18 mutants were obtained from both species. The neutral lipid evaluation on said 18 mutants allowed the retention of only 7 to further fatty acid characterization. Finally, gas chromatography revealed that the mutant 5c Isochrysis sp. was characterized by a high level of saturated fatty acids (52.3%), higher amount of monounsaturated fatty acids (29.3%), lower level of polyunsaturated fatty acids (18.4%) and a significant 1.3-fold increase in its C16-C18 content compared to the wild-type strain, which would make it an interesting candidate for biofuel production.
机译：微藻作为第三代生物燃料的替代品，对于未来的生物柴油生产可能具有重要意义。为了选择最适合生产生物柴油的菌株，对从突尼斯海岸分离的三种微藻物种，即等鞭藻属，纳氏绿藻和特氏藻进行了生物化学表征。最初，气相色谱分析表明，等鞭藻属。和N. maritima包含的总脂肪酸分别比Tetraselmis sp多5到10倍。然后，两个微藻等鞭藻。使用紫外-C辐射对海马猪笼草和海马猪笼草进行随机诱变。随后，从这两个物种总共获得了18个突变体。对所述18个突变体的中性脂质评估仅允许保留7个以进一步表征脂肪酸。最后，气相色谱显示突变体5c Isochrysis sp。其特点是饱和脂肪酸含量高（52.3％），单不饱和脂肪酸含量更高（29.3％），多不饱和脂肪酸含量更低（18.4％）和其C16-C18含量显着增加1.3倍野生型菌株，这将使其成为生物燃料生产的有趣候选者。

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