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首页> 外文期刊>Bioprocess and Biosystems Engineering >Production of carotenoids and lipids by Rhodococcus opacus PD630 in batch and fed-batch culture
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Production of carotenoids and lipids by Rhodococcus opacus PD630 in batch and fed-batch culture

机译：分批和补料分批培养的不透明红球菌PD630生产类胡萝卜素和脂质

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摘要

Production of carotenoids by Rhodococcus opacus PD630 is reported. A modified mineral salt medium formulated with glycerol as an inexpensive carbon source was used for the fermentation. Ammonium acetate was the nitrogen source. A dry cell mass concentration of nearly 5.4 g/L could be produced in shake flasks with a carotenoid concentration of 0.54 mg/L. In batch culture in a 5 L bioreactor, without pH control, the maximum dry biomass concentration was similar to 30 % lower than in shake flasks and the carotenoids concentration was 0.09 mg/L. Both the biomass concentration and the carotenoids concentration could be raised using a fed-batch operation with a feed mixture of ammonium acetate and acetic acid. With this strategy, the final biomass concentration was 8.2 g/L and the carotenoids concentration was 0.20 mg/L in a 10-day fermentation. A control of pH proved to be unnecessary for maximizing the production of carotenoids in this fermentation.
机译：据报道，不透明红球菌PD630可产生类胡萝卜素。用甘油作为廉价碳源配制的改良矿物盐培养基用于发酵。乙酸铵是氮源。在摇瓶中，类胡萝卜素浓度为0.54 mg / L，可以产生近5.4 g / L的干细胞质量浓度。在没有pH值控制的5 L生物反应器中分批培养时，最大干生物量浓度比摇瓶中低约30％，类胡萝卜素浓度为0.09 mg / L。使用乙酸铵和乙酸的进料混合物的分批进料操作可以提高生物质浓度和类胡萝卜素浓度。通过这种策略，在10天的发酵过程中，最终生物质浓度为8.2 g / L，类胡萝卜素浓度为0.20 mg / L。事实证明，为了使该发酵过程中的类胡萝卜素产量最大化，控制pH值是不必要的。

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