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Effect of volatile fatty acids mixtures on the simultaneous photofermentative production of hydrogen and polyhydroxybutyrate

机译:挥发性脂肪酸混合物对氢和多羟基丁酸酯同时光发酵产生的影响

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摘要

Purple non-sulfur bacteria generate hydrogen and polyhydroxybutyrate (PHB) as a mechanism for disposing of reducing equivalents generated during substrate consumption. However, both pathways compete for the reducing equivalents released from bacteria growing under certain substrates, thus the formation of hydrogen or PHB is detrimental to the formation of each other. The effect of mixtures of acetic, propionic and butyric acids on the formation of H-2 and PHB was evaluated using BoxBehnken design. A bacterial community mainly constituted by Rhodopseudomonas palustris was used as inoculum. It was observed that the three volatile fatty acids had a significant effect on the specific PHB production. However, only the propionic acid had a significant effect on the specific H-2 production activity and the highest value was observed when acetate was the main component in the mixture. The maximum values for the specific PHB and hydrogen production rates were 16.4 mg-PHB/g-TSS/day and 391 mL-H-2/g-TSS/day, respectively.
机译:紫色的非硫细菌会产生氢和聚羟基丁酸酯(PHB),作为处理减少底物消耗过程中产生的当量的机制。然而,这两种途径都竞争从在某些底物下生长的细菌释放的还原当量,因此氢或PHB的形成不利于彼此的形成。使用BoxBehnken设计评估了乙酸,丙酸和丁酸混合物对H-2和PHB形成的影响。使用主要由Phodopseudomonas palustris组成的细菌群落作为接种物。观察到三种挥发性脂肪酸对特定的PHB产生有显着影响。但是,只有丙酸对H-2的比生产活性有显着影响,而当乙酸盐是混合物中的主要成分时,则观察到最大值。特定PHB和氢气产生速率的最大值分别为16.4 mg-PHB / g-TSS /天和391 mL-H-2 / g-TSS /天。

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