• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >A kinetic model for flavonoid production in tea cell culture
【24h】

A kinetic model for flavonoid production in tea cell culture

机译：茶细胞培养中黄酮生成的动力学模型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

As one of the strategies for efficient production of a metabolite from cell cultures, a kinetic model is very useful tool to predict productivity under various culture conditions. In this study, we propose a kinetic model for flavonoid production in tea cell culture based on the cell life cycle and expression of PAL, the gene encoding phenylalanine ammonia-lyase (PAL)-the key enzyme in flavonoid biosynthesis. The flavonoid production rate was considered to be related to the amount of active PAL. Synthesis of PAL was modelled based on a general gene expression/translation mechanism, including the transcription of DNA encoding PAL into mRNA and the translation of PAL mRNA into the PAL protein. The transcription of DNA was assumed to be promoted at high light intensity and suppressed by a feedback regulatory mechanism at high flavonoid concentrations. In the model, mRNA and PAL were considered to self-decompose and to be lost by cell rupture. The model constants were estimated by fitting the experimental results obtained from tea cell cultures under various light intensities. The model accurately described the kinetic behaviors of dry and fresh cell concentrations, glucose concentration, cell viability, PAL specific activity, and flavonoid content under a wide range of light intensities. The model simulated flavonoid productivity per medium under various culture conditions. Therefore, this model will be useful to predict optimum culture conditions for maximum flavonoid productivity in cultured tea cells.
机译：作为从细胞培养物中高效生产代谢产物的策略之一，动力学模型是预测各种培养条件下生产力的非常有用的工具。在这项研究中，我们基于细胞生命周期和PAL的表达，提出了茶细胞培养中类黄酮生成的动力学模型，PAL是编码类黄酮生物合成中的关键酶苯丙氨酸解氨酶（PAL）的基因。类黄酮产率被认为与活性PAL的量有关。基于一般的基因表达/翻译机制，包括将编码PAL的DNA转录成mRNA和将PAL mRNA翻译成PAL蛋白，对PAL的合成进行建模。假定DNA的转录在高光强度下被促进，并在高黄酮类化合物浓度下受到反馈调节机制的抑制。在该模型中，mRNA和PAL被认为会自我分解并因细胞破裂而丢失。通过拟合在各种光强度下从茶细胞培养物中获得的实验结果来估计模型常数。该模型准确地描述了在各种光强度下干细胞和新鲜细胞的动力学行为，葡萄糖浓度，细胞生存力，PAL比活性和类黄酮含量。该模型模拟了各种培养条件下每种培养基的类黄酮生产率。因此，该模型将有助于预测最佳培养条件，从而在培养的茶细胞中获得最大的类黄酮生产率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号