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Enzymatic synthesis of ascorbyl ester derived from linoleic acid

机译：酶法合成亚油酸抗坏血酸酯

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摘要

Antioxidants are substances that defend cells against damage, kidnapping and destroying free radicals. They have been largely used in the food industry due the possibility to control the oxidation process, aimed to increase shelf life. Thus, esterification reaction to obtain ascorbyl linoleate catalyzed by Novozym 435 lipase assisted by ultrasound bath was investigated. In this work, molecular sieve (4 angstrom) was added to the reaction medium to remove the water formed during the esterification reaction to improve the process performance. According to the results, ascorbyl linoleate production up to 90 % was reached after 1 h of reaction time carried out using ultrasound bath, 1: 9 molar ratio of substrates L-ascorbic acid to linoleic acid, 20 mL of tert-butanol as organic solvent, 5 wt% of Novozym 435 lipase, 10 wt% of molecular sieve at 70 degrees C.
机译：抗氧化剂是保护细胞免受损害，绑架和破坏自由基的物质。由于可以控制氧化过程以延长保存期限，因此它们已广泛用于食品工业。因此，研究了在超声浴辅助下，Novozym 435脂肪酶催化酯化反应得到抗坏血酸亚油酸酯。在这项工作中，将分子筛（4埃）添加到反应介质中，以除去在酯化反应过程中形成的水​​，从而改善工艺性能。根据结果​​，使用超声波浴，在1小时的底物L-抗坏血酸与亚油酸的摩尔比，20 mL的叔丁醇作为有机溶剂进行反应1小时后，达到了90％的抗坏血酸亚油酸酯产量，5重量％的Novozym 435脂肪酶，10重量％的分子筛在70摄氏度下。

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