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Use of saline waste water from demineralization of cheese whey for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4

机译:干酪乳清脱盐中的盐分废水用于培养裂殖壶菌PA-968和海涂Japonochytrium AN-4

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Saline waste water from demineralization of cheese whey was used as the main component of waste saline medium (WSM) for cultivation of thraustochytrids. The suitability of WSM for cultivation of Schizochytrium limacinum PA-968 and Japonochytrium marinum AN-4 was evaluated by comparison with cultivation on nutrient medium (NM) in shake flask and fermenter cultures. Biomass productivities achieved in WSM for the thraustochytrids were comparable with those in NM for both shake flask and fermenter cultures. The maximum total lipid content (56.71% dry cell weight) and docosahexaenoic acid productivity (0.86 g/L/day) were achieved by J. marinum AN-4 grown on WSM in shake flask and fermenter cultures, respectively. A cost estimate of WSM suggests that this medium could result in lower production costs for thraustochytrid biomass and lipids and contribute to the effective reduction in saline diary process waste water.
机译:干酪乳清脱盐所产生的盐水被用作废破囊壶菌培养的废盐水培养基(WSM)的主要成分。通过与摇瓶和发酵罐培养液中营养培养基(NM)的培养进行比较,评估了WSM在甘蔗裂殖壶菌PA-968和海生Japonochytrium marinum AN-4上的适宜性。在WSM中,破囊壶菌的生物量生产率与摇瓶和发酵罐培养物的NM相当。最大的总脂质含量(56.71%干细胞重量)和二十二碳六烯酸生产率(0.86 g / L /天)分别通过在摇瓶和发酵罐培养物中于WSM上生长的海生J. marinum AN-4获得。 WSM的成本估算表明,该介质可以降低破囊壶菌生物量和脂质的生产成本,并有助于有效减少盐业日记工艺废水。

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