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首页> 外文期刊>Biotechnology Letters >Heterotrophic cultivation of Chlorella vulgaris using saline waste water from the demineralization of cheese whey
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Heterotrophic cultivation of Chlorella vulgaris using saline waste water from the demineralization of cheese whey

机译：奶酪乳清脱矿水中盐水废水的纯养殖栽培

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摘要

Objective Desalination of cheese whey by electrodialysis yields saline wastewater (SWW). The goal was to test this as the basis of a culture medium and to prove experimentally the concept that it was a suitable resource for heterotrophic cultivation of the freshwater green microalga Chlorella vulgaris. Results Optimization of glucose concentration, nitrogen source and medium salinity for microalgal growth was first carried out in defined medium (DM) and shake flasks. These results were then adopted in shake flask cultivation experiments using pre-treated SWW medium (PSWW). Subsequently, microalgal growth under optimized conditions was tested in bioreactors. Various media such as DM, PSWW and diluted PSWW (DPSWW) were compared. Volumetric biomass productivities decreased in the order DM (0.371 g L-1 h(-1), urea) > DPSWW (0.315 g L-1 h(-1), soy peptone) > PSWW (0.152 g L-1 h(-1), soy peptone). Although biomass productivities in DPSWW and PSWW media were significantly lower than in DM, these media required the addition of only 66 and 33% of DM N sources, respectively. No other added DM component was necessary in (D)PSWW to achieve microalgal growth. Conclusions Although the optimized cultivation of freshwater microalgae on alternative medium based on SWW resulted in biomass productivities lower than those on DM, the required addition of N sources was also lower. Potentially lower production costs of Chlorella biomass and the meaningful use of SWW are the main outcomes of this work.
机译：电渗析乳酪乳清乳清的客观脱盐产生盐水废水（SWW）。目标是将此作为文化媒体的基础，并通过实验证明这是一种淡水绿色微藻小黄菌的异养培养的合适资源。结果在定义的培养基（DM）和摇瓶中首先进行微良生长的葡萄糖浓度，氮源和中盐度的优化。然后使用预处理的SWW培养基（PSWW）在摇瓶培养实验中采用这些结果。随后，在生物反应器中测试优化条件下的微藻生长。比较各种媒体，如DM，PSWW和稀释的PSWW（DPSWW）。量化DM（0.371g L-1 H（-1），尿素）> dpsww（0.315g l-1 h（-1），大豆蛋白胨）> psww（0.152g l-1 h（ - 1），大豆蛋白胨）。尽管DPSWW和PSWW介质中的生物质生产率显着低于DM，但这些介质可以分别添加仅66％和33％的DM N源。在（d）psww中没有其他添加的DM组分以实现微藻生长。结论虽然基于SWW的替代介质上的淡水微藻的优化培养导致生物质生产率低于DM的生物量产态，但是N个源的所需添加也较低。可能降低小球藻生物质的生产成本，并且SWW的有意义使用是这项工作的主要结果。

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