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首页> 外文期刊>Bioprocess and Biosystems Engineering >Combined characterization of bovine polyhemoglobin microcapsules by UV-Vis absorption spectroscopy and cyclic voltammetry
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Combined characterization of bovine polyhemoglobin microcapsules by UV-Vis absorption spectroscopy and cyclic voltammetry

机译：牛多血红蛋白微胶囊的紫外-可见吸收光谱和循环伏安法联合表征

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摘要

Polyhemoglobin produced from pure bovine hemoglobin by reaction with PEG bis(N-succynimidil succinate) as a cross-linking agent was encapsulated in gelatin and dehydrated by freeze-drying. Free carboxyhemoglobin and polyhemoglobin microcapsules were characterized by UV-Vis spectroscopy in the absorption range 450-650 nm and cyclic voltammetry in the voltage range from -0.8 to 0.6 mV to evaluate the ability to break the bond with carbon monoxide and to study the carrier's affinity for oxygen, respectively. SEM used to observe the shape of cross-linked gelatin-polyhemoglobin microparticles showed a regular distribution of globular shapes, with mean size of similar to 750 nm, which was ascribed to gelatin. Atomic absorption spectroscopy was also performed to detect iron presence in microparticles. Cyclic voltammetry using an Ag-AgCl electrode highlighted characteristic peaks at around -0.6 mV that were attributed to reversible oxygen bonding with iron in oxy-polyhemoglobin structure. These results suggest this technique as a powerful, direct and alternative method to evaluate the extent of hemoglobin oxygenation.
机译：由纯牛血红蛋白通过与作为交联剂的PEG双（N-蔗糖酰胺基琥珀酸酯）反应而产生的多血红蛋白被封装在明胶中，并通过冷冻干燥进行脱水。通过在450-650 nm的吸收范围内的UV-Vis光谱和在-0.8至0.6 mV的电压范围内的循环伏安法表征游离的羧基血红蛋白和聚血红蛋白微胶囊，以评估与一氧化碳之间的键断裂能力以及研究载体的亲和力分别用于氧气。用于观察交联的明胶-聚血红蛋白微粒的形状的SEM显示出规则的球状分布，平均大小类似于750 nm，这归因于明胶。还进行了原子吸收光谱法以检测微粒中铁的存在。使用Ag-AgCl电极的循环伏安法突出显示了-0.6 mV附近的特征峰，该峰归因于氧-聚血红蛋白结构中与铁的可逆氧键合。这些结果表明，该技术是评估血红蛋白氧合程度的有效，直接和替代方法。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第3期|431-438|共8页
  • 作者

    Knirsch Marcos Camargo; DellAnno Filippo; Salerno Marco; Larosa Claudio; Polakiewicz Bronislaw; Eggenhoffner Roberto; Converti Attilio;

  • 作者单位

    Univ Genoa, Dept Civil Chem & Environm Engn, Via Opera Pia 15, I-16145 Genoa, Italy|Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Ave Prof Lineu Prestes 580,Bloco 16, BR-05508900 Sao Paulo, SP, Brazil;

    Univ Genoa, Dept Civil Chem & Environm Engn, Via Opera Pia 15, I-16145 Genoa, Italy;

    Ist Italiano Tecnol, Dept Nanophys, Via Morego 30, I-16163 Genoa, Italy;

    Univ Genoa, Dept Civil Chem & Environm Engn, Via Opera Pia 15, I-16145 Genoa, Italy;

    Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Ave Prof Lineu Prestes 580,Bloco 16, BR-05508900 Sao Paulo, SP, Brazil;

    Univ Genoa, Dept Surg Sci & Integrate Diagnost, Corso Europa 30, I-16145 Genoa, Italy|INBB, Viale Medaglie DOro 305, I-00136 Rome, Italy;

    Univ Genoa, Dept Civil Chem & Environm Engn, Via Opera Pia 15, I-16145 Genoa, Italy;

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  • 正文语种 eng
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  • 关键词

    Cyclic voltammetry; Freeze-drying; Polyhemoglobin; Microencapsulation; Oxygen carrier;

    机译：循环伏安法;冷冻干燥;多血红蛋白;微囊化;载氧剂;

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