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首页> 外文期刊>Bioprocess and Biosystems Engineering >Determination of kinetics and heat of hydrolysis for non-homogenous substrate by isothermal calorimetry
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Determination of kinetics and heat of hydrolysis for non-homogenous substrate by isothermal calorimetry

机译：等温量热法测定非均质底物的水解动力学和水解热

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摘要

The competitiveness of the second-generation bioethanol by biotechnological process requires an effective and quantitative control of biochemical reactions. In this study, the potential of isothermal calorimetry technique to measure heat and kinetics of a non-homogeneous substrate enzymatic hydrolysis is intended. Using this technique, optimum temperature of the enzymes used for lignocellulosic molecules hydrolysis was determined. Thus, the amount of substrate-to-enzyme ratio was highlighted as an important parameter of the hydrolysis yield. Furthermore, a new enzymes' cocktail efficiency consisting of a mix of cellulases and cellobiose dehydrogenase (CDH) was qualified by this technique. The results showed that this cocktail allowed the production of a high amount of gluconic acid that could improve the attractiveness of these second-generation biofuels. From the set of experiments, the hydrolysis heat of wheat straw was derived and a meaningful value of -32.2 +/- 3.2 J g(-1) (gram reducing sugars product) is calculated. Then, isothermal measurements were used to determine kinetic constants of the cellulases and CDH mix on wheat straw. Results showed that this enzyme cocktail has an optimal rate at 45 A degrees C in the range of temperatures tested (40-55 A degrees C).
机译：第二代生物乙醇通过生物技术过程的竞争力要求对生化反应进行有效和定量的控制。在这项研究中，打算用等温量热技术来测量非均质底物酶水解的热和动力学。使用该技术，确定了用于木质纤维素分子水解的酶的最佳温度。因此，底物与酶之比的量被强调为水解产量的重要参数。此外，通过该技术验证了由纤维素酶和纤维二糖脱氢酶（CDH）组成的新酶的鸡尾酒效率。结果表明，这种鸡尾酒可以产生大量的葡糖酸，可以提高这些第二代生物燃料的吸引力。从一组实验中，得出了麦草的水解热，并计算了有意义的值-32.2 +/- 3.2 J g（-1）（克还原糖产品）。然后，使用等温测量来确定纤维素酶和CDH混合物在麦草上的动力学常数。结果表明，该酶混合物在测试温度范围（40-55 A摄氏度）下，在45 A摄氏度下具有最佳速率。

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