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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enantioselective resolution of racemic flurbiprofen methyl ester by lipase encapsulated mercapto calix[4]arenes capped Fe3O4 nanoparticles
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Enantioselective resolution of racemic flurbiprofen methyl ester by lipase encapsulated mercapto calix[4]arenes capped Fe3O4 nanoparticles

机译：脂肪酶包裹巯基杯[4]芳烃封端的Fe3O4纳米颗粒的外消旋氟比洛芬甲酯的对映选择性拆分

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摘要

This work presents the synthesis of new mercapto calix[4]arenes derivatives (4 and 5). These derivatives were capped on Fe3O4 magnetic nanoparticles and subsequently encapsulated with Candida rugosa through sol-gel method to furnish enc-4 and enc-5, respectively, to enhance catalytic activity and enantioselectivity of lipase for hydrolysis reaction of racemic flurbiprofen methyl ester. Catalytic activity and enantioselectivity of enc-4 and enc-5 were assayed at different pH and temperature conditions and it was found that the resultant encapsulated enzyme exhibited higher thermal and operational stabilities compared to the free lipase in which enc-5 showed the excellent rate of enantioselectivity (E = 176) for S-flurbiprofen better than free lipase (E = 137) at pH 7 and 35 A degrees C for 48 h. The time study shows that enantioselectivity reached the maximum value of E = 244 after 72 h. Catalytic activity of these materials was hardly affected by 20 and 23% after five usages of enc-4 and enc-5, respectively.
机译：这项工作提出了新的巯基杯[4]芳烃衍生物（4和5）的合成。将这些衍生物封端在Fe3O4磁性纳米颗粒上，然后通过溶胶-凝胶法用念珠菌包裹，分别提供enc-4和enc-5，以增强脂肪酶对外消旋氟比洛芬甲酯水解反应的催化活性和对映选择性。在不同的pH和温度条件下对enc-4和enc-5的催化活性和对映选择性进行了测定，发现与enc-5表现出优良的游离脂肪酶的游离脂肪酶相比，所得包封的酶表现出更高的热稳定性和操作稳定性。在pH 7和35 A的温度下48小时，S-氟比洛芬的对映选择性（E = 176）优于游离脂肪酶（E = 137）。时间研究表明，对映选择性在72小时后达到E = 244的最大值。分别使用enc-4和enc-5后，这些材料的催化活性几乎不受20％和23％的影响。

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