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首页> 外文期刊>Bioprocess and Biosystems Engineering >Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26
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Sucrose fed-batch strategy enhanced biomass, polysaccharide, and ganoderic acids production in fermentation of Ganoderma lucidum 5.26

机译：蔗糖补料分批策略提高了灵芝5.26发酵中生物量，多糖和灵芝酸的产量

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摘要

Ganoderma, as a Chinese traditional medicine, has multiple bioactivities. However, industrial production was limited due to low yield during Ganoderma fermentation. In this work, sucrose was found to greatly enhance intracellular polysaccharide (IPS) content and specific extracellular polysaccharide (EPS) production rate. The mechanism was studied by analyzing the activities of enzymes related to polysaccharide biosynthesis. The results revealed that sucrose regulated the activities of phosphoglucomutase and phosphoglucose isomerase. When glucose and sucrose mixture was used as carbon source, biomass, polysaccharide and ganoderic acids (GAs) production was greatly enhanced. A sucrose fed-batch strategy was developed in 10-L bioreactor, and was scaled up to 300-L bioreactor. The biomass, EPS and IPS production was 25.5, 2.9 and 4.8 g/L, respectively, which was the highest biomass and IPS production in pilot scale. This study provides information for further understanding the regulation mechanism of Ganoderma polysaccharide biosynthesis. It demonstrates that sucrose fed-batch is a useful strategy for enhancing Ganoderma biomass, polysaccharide and GAs production.
机译：灵芝作为一种中药，具有多种生物活性。但是，由于灵芝发酵过程中产量低，工业生产受到限制。在这项工作中，发现蔗糖极大地提高了细胞内多糖（IPS）的含量和特定的细胞外多糖（EPS）的生产率。通过分析与多糖生物合成有关的酶的活性研究了该机理。结果表明，蔗糖调节磷酸葡萄糖变位酶和磷酸葡萄糖异构酶的活性。当使用葡萄糖和蔗糖混合物作为碳源时，生物量，多糖和灵芝酸（GAs）的产量大大提高。在10升生物反应器中开发了蔗糖补料分批策略，并将其规模扩大到300升生物反应器。生物质，EPS和IPS产量分别为25.5、2.9和4.8 g / L，是中试规模的最高生物质和IPS产量。该研究为进一步了解灵芝多糖生物合成的调控机制提供了信息。它表明蔗糖补料分批法是提高灵芝生物量，多糖和GA产生的有用策略。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第1期|37-44|共8页
  • 作者

    Wei Zhen-hua; Liu Lianliang; Guo Xiao-feng; Li Yan-jun; Hou Bao-chao; Fan Qiu-ling; Wang Kai-xiang; Luo Yingdi; Zhong Jian-Jiang;

  • 作者单位

    Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China|Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China;

    Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Hangzhou Wahaha Grp Co Ltd, Inst Bioengn, Zhejiang Prov Key Lab Food Biotechnol, Hangzhou 310018, Zhejiang, Peoples R China;

    Zhejiang Univ, Dept Chem & Biochem Engn, Hangzhou 310027, Zhejiang, Peoples R China;

    Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China|Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ganoderma; Fed-batch; Fermentation; Polysaccharide; Ganoderic acids;

    机译：灵芝;补料分批;发酵;多糖;灵芝酸;

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