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首页> 外文期刊>Bioprocess and Biosystems Engineering >Impact of milling, enzyme addition, and steam explosion on the solid waste biomethanation of an olive oil production plant
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Impact of milling, enzyme addition, and steam explosion on the solid waste biomethanation of an olive oil production plant

机译：研磨，添加酶和蒸汽爆炸对橄榄油生产厂固体废弃物生物甲烷化的影响

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摘要

Anaerobic digestion is a consolidated bioprocess which can be further enhanced by incorporating an upstream pretreatment unit. The olive oil production produces a large amount of solid waste which needs to be properly managed and disposed. Three different pretreatment techniques were evaluated in regard to their impact on the anaerobic biodegradability: manual milling of olive pomace (OP), enzyme maceration, direct enzyme addition, and thermal hydrolysis of two-phase olive mill waste. The Gompertz equation was used to obtain parameters for comparison purposes. A substrate/inoculum ratio 0.5 was found to be the best to be used in anaerobic batch test with olive pomace as substrate. Mechanical pretreatment of OP by milling increases the methane production rate while keeping the maximum methane yield. The enzymatic pretreatment showed different results depending on the chosen pretreatment strategies. After the enzymatic maceration pretreatment, a methane production of 274 ml CH4 g VS (added) (-1) was achieved, which represents an improvement of 32 and 71 % compared to the blank and control, respectively. The direct enzyme addition pretreatment showed no improvement in both the rate and the maximum methane production. Steam explosion showed no improvement on the anaerobic degradability of two-phase olive mill waste; however, thermal hydrolysis with no rapid depressurization enhanced notoriously both the maximum rate (50 %) and methane yield (70 %).
机译：厌氧消化是一个合并的生物过程，可以通过合并上游预处理单元来进一步增强。橄榄油生产会产生大量的固体废物，需要妥善管理和处置。评估了三种不同的预处理技术对厌氧生物降解性的影响：橄榄果渣（OP）的手动研磨，酶浸渍，直接酶添加以及两相橄榄研磨机废料的热水解。使用Gompertz方程获取参数以进行比较。发现在以橄榄果渣为底物的厌氧分批试验中，最佳的底物/接种物比率为0.5。通过研磨对OP进行机械预处理可以提高甲烷产生速率，同时保持最大甲烷产量。取决于选择的预处理策略，酶促预处理显示出不同的结果。酶浸渍预处理后，甲烷产量为274 ml CH4 g VS（添加）（-1），与空白对照相比，分别提高了32％和71％。直接添加酶预处理未显示出速率和最大甲烷产量的改善。蒸汽爆炸表明两相橄榄磨机废料的厌氧降解性没有改善。然而，没有快速降压的热水解显着提高了最大速率（50％）和甲烷产率（70％）。

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