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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system
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Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system

机译：在实验室规模的系统中使用内部纤维素酶在高固含量下增强弱碱碱预处理稻草的酶促水解

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摘要

In the present study, scale-up systems for cellulase production and enzymatic hydrolysis of pre-treated rice straw at high-solid loadings were designed, fabricated and tested in the laboratory. Cellulase production was carried out using tray fermentation at 45 degrees C by Aspergillus terreus in a temperature-controlled humidity chamber. Enzymatic hydrolysis studies were performed in a horizontal rotary drum reactor at 50 degrees C with 25 % (w/v) solid loading and 9 FPU g(-1) substrate enzyme load using inhouse as well commercial cellulases. Highly concentrated fermentable sugars up to 20 % were obtained at 40 h with an increased saccharification efficiency of 76 % compared to laboratory findings (69.2 %). These findings demonstrate that we developed a simple and less energy intensive bench scale system for efficient high-solid saccharification. External supplementation of commercial beta-glucosidase and hemicellulase ensured better hydrolysis and further increased the saccharification efficiency by 14.5 and 20 %, respectively. An attempt was also made to recover cellulolytic enzymes using ultrafiltration module and nearly 79-84 % of the cellulases and more than 90 % of the sugars were recovered from the saccharification mixture.
机译：在本研究中，在实验室中设计，制造和测试了在高固含量下预处理纤维素酶生产和酶解水解的放大系统。纤维素酶的生产是在温度控制的湿度箱中使用曲霉菌在45摄氏度下通过盘式发酵进行的。酶促水解研究是在水平旋转鼓式反应器中使用内部以及商业纤维素酶在50°C，25％（w / v）固体负载和9 FPU g（-1）底物酶负载的条件下进行的。与实验室检查结果（69.2％）相比，在40h时获得了高达20％的高浓度可发酵糖，糖化效率提高了76％。这些发现表明，我们开发了一种简单且耗能较少的台式规模系统，以进行高效的高固体糖化。外部补充商业性β-葡萄糖苷酶和半纤维素酶可确保更好的水解效果，并分别将糖化效率提高14.5％和20％。还尝试使用超滤模块回收纤维素分解酶，并且从糖化混合物中回收了近79-84％的纤维素酶和90％以上的糖。

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