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首页> 外文期刊>Bioprocess and Biosystems Engineering >Phosphate limitation promotes unsaturated fatty acids and arachidonic acid biosynthesis by microalgae Porphyridium purpureum
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Phosphate limitation promotes unsaturated fatty acids and arachidonic acid biosynthesis by microalgae Porphyridium purpureum

机译：限制磷酸盐通过微藻紫菜促进了不饱和脂肪酸和花生四烯酸的生物合成

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摘要

Polyunsaturated fatty acids (PUFAs) are highly appreciated on their nutritive value for human health and aquaculture. P. purpureum, one of the red microalgae acknowledged as a promising accumulator of ARA, was chosen as the target algae in the present research. Effects of sodium bicarbonate (0.04-1.2 g/L), temperature (25, 30 and 33 degrees C) and phosphate (0.00-0.14 g/L) on biomass yield, total fatty acids (TFA) and arachidonic acid (ARA) accumulation were investigated systemically. NaHCO3 dose of 0.8 g/L and moderate temperature of 30 degrees C were preferred. In addition, TFA and ARA production were significantly enhanced by an appropriate concentration of phosphate, and the highest TFA yield of 666.38 mg/L and ARA yield of 159.74 mg/L were obtained at a phosphate concentration of 0.035 g/L. Interestingly, with phosphate concentration continuing to fall, UFA/TFA and ARA/EPA ratios were increased accordingly, suggesting that phosphate limitation promoted unsaturated fatty acids and arachidonic acid biosynthesis. Low concentration of phosphate may be favored to increase the enzymatic activities of Delta 6-desaturase, which played a key role in catalyzing the conversion of C16:0 to C18:2, and thus the selectivity of UFA increased. Meanwhile, the increase of ARA selectivity could be attributed to omega 6 pathway promotion and Delta 17-desaturase activity inhibition with phosphate limitation. Phosphate limitation strategy enhanced unsaturated fatty acids and ARA biosynthesis in P. purpureum, and can be applied in commercial scale manufacturing and commercialization of ARA.
机译：多不饱和脂肪酸（PUFA）对人类健康和水产养殖的营养价值受到高度赞赏。在本研究中，P。purpureum被认为是有希望的ARA的红色微藻之一，被选为目标藻类。碳酸氢钠（0.04-1.2 g / L），温度（25、30和33摄氏度）和磷酸盐（0.00-0.14 g / L）对生物量产量，总脂肪酸（TFA）和花生四烯酸（ARA）积累的影响被系统地调查。 NaHCO3剂量为0.8 g / L，温度为30摄氏度为宜。此外，适当的磷酸盐浓度可显着提高TFA和ARA的产量，而磷酸盐浓度为0.035 g / L时，TFA的最高产量为666.38 mg / L，ARA的最高产量为159.74 mg / L。有趣的是，随着磷酸盐浓度的持续下降，UFA / TFA和ARA / EPA的比例也相应增加，表明磷酸盐限制促进了不饱和脂肪酸和花生四烯酸的生物合成。低浓度的磷酸盐可能有利于增加Delta 6-去饱和酶的酶活性，这在催化C16：0到C18：2的转化中起关键作用，因此UFA的选择性增加。同时，ARA选择性的提高可能归因于omega 6途径的促进和具有磷酸盐限制的Delta 17-去饱和酶活性的抑制。磷酸盐限制策略增强了紫菜中的不饱和脂肪酸和ARA的生物合成，可用于ARA的商业规模生产和商业化。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第7期|1129-1136|共8页
  • 作者

    Su Gaomin; Jiao Kailin; Li Zheng; Guo Xiaoyi; Chang Jingyu; Ndikubwimana Theoneste; Sun Yong; Zeng Xianhai; Lu Yinghua; Lin Lu;

  • 作者单位

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Dept Chem & Biochem Engn, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China|Xiamen Univ, Key Lab Synthet Biotechnol Xiamen City, Xiamen 361005, Peoples R China;

    Xiamen Univ, Dept Chem & Biochem Engn, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China|Xiamen Univ, Key Lab Synthet Biotechnol Xiamen City, Xiamen 361005, Peoples R China;

    Xiamen Univ, Coll Energy, Xiamen 361005, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Porphyridium purpureum; Phosphate limitation; Total fatty acids; Arachidonic acid;

    机译：紫菜卟啉;磷酸盐限制;总脂肪酸;花生四烯酸;

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