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首页> 外文期刊>Bioprocess and Biosystems Engineering >Molecular mechanism of arachidonic acid biosynthesis in Porphyridium purpureum promoted by nitrogen limitation
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Molecular mechanism of arachidonic acid biosynthesis in Porphyridium purpureum promoted by nitrogen limitation

机译：氮素抑制促进卟啉毒性卟啉毒素中的花生酸生物合成的分子机制

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摘要

The red alga Porphyridium purpureum has been known to produce polyunsaturated fatty acids, especially arachidonic acid (ARA), under stressful conditions. However, there is no consistent conclusion about the response of ARA in this alga to nitrogen (N) stress. Also, no research has been done to clearly elucidate the underlying molecular mechanisms of N stress. In this work, P. purpureum CoE1 was cultivated under nitrogen limitation conditions and the putative Delta 5-desaturase related gene FADSD5 was isolated. The results showed that the fatty acids in P. purpureum CoE1 were significantly higher in the N limited cultures (54.3 mg g(-1)) than in the N-replete cultures (45.3 mg g(-1)) at the 18th day (t-test, p 0.001), which was attributed to the upregulated abundance of the putative Delta 5-desaturase related protein, Delta 5-Des. The study also indicated that the expression of the putative Delta 5-desaturase related gene, FADSD5, increased with cell growth, demonstrating considerable potentials for ARA biosynthesis in P. purpureum CoE1. These results might guide the direction in illuminating the biosynthetic pathway of fatty acids with molecular evidence and enable genetic modifications of P. purpureum CoE1 for enhancing the ARA accumulation.
机译：已知红藻卟啉尿卟啉在压力条件下产生多不饱和脂肪酸，特别是花生酸（ARA）。然而，关于该藻ARA对氮气（n）应激的响应没有一致的结论。此外，没有进行研究以清楚地阐明N应激的潜在分子机制。在这项工作中，在氮气限制条件下培养P. purpureum Coe1，并分离推定的delta 5-去饱和酶相关基因FADSD5。结果表明，在第18天（45.3mg）（45.3mg）（45.3mg（-1））中，P. purpureum Coe1中的脂肪酸显着高于第18天（45.3mg（-1））（ T-试验，P <0.001），其归因于推定δ5-去饱和酶相关蛋白，Delta 5-des的上调丰度。该研究还表明，调用δ5-去饱和酶相关基因的表达，FADSD5增加了细胞生长，展示了P.Purpureum CoE1中ARA生物合成的相当大的潜力。这些结果可能导致在分子证​​据照射脂肪酸生物合成途径的方向，并使P. purpureum Coe1的遗传修饰提高ARA积累。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2021年第7期|1491-1499|共9页
  • 作者

    Jiao Kailin; Xiao Wupeng; Shi Xingguo; Ho Shih-Hsin; Chang Jo-Shu; Ng I-Son; Tang Xing; Sun Yong; Zeng Xianhai; Lin Lu;

  • 作者单位

    Xiamen Univ Coll Energy Xiamen 361102 Peoples R China|Minnan Normal Univ Coll Chem & Environm Zhangzhou 363000 Peoples R China;

    Xiamen Univ Coll Environm & Ecol Fujian Prov Key Lab Coastal Ecol & Environm Studi State Key Lab Marine Environm Sci Xiamen 361102 Peoples R China;

    Fuzhou Univ Coll Biol Sci & Engn Fujian 350116 Peoples R China;

    Harbin Inst Technol Sch Environm State Key Lab Urban Water Resource & Environm Harbin 150006 Peoples R China;

    Natl Cheng Kung Univ Dept Chem Engn Tainan 70101 Taiwan;

    Natl Cheng Kung Univ Dept Chem Engn Tainan 70101 Taiwan;

    Xiamen Univ Coll Energy Xiamen 361102 Peoples R China|Xiamen Univ Xiamen Key Lab Clean & High Valued Convers Techno Fujian Engn & Res Ctr Clean & High Valued Convers Xiamen 361102 Peoples R China;

    Xiamen Univ Coll Energy Xiamen 361102 Peoples R China|Xiamen Univ Xiamen Key Lab Clean & High Valued Convers Techno Fujian Engn & Res Ctr Clean & High Valued Convers Xiamen 361102 Peoples R China;

    Xiamen Univ Coll Energy Xiamen 361102 Peoples R China|Xiamen Univ Xiamen Key Lab Clean & High Valued Convers Techno Fujian Engn & Res Ctr Clean & High Valued Convers Xiamen 361102 Peoples R China;

    Xiamen Univ Coll Energy Xiamen 361102 Peoples R China|Xiamen Univ Xiamen Key Lab Clean & High Valued Convers Techno Fujian Engn & Res Ctr Clean & High Valued Convers Xiamen 361102 Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Porphyridium purpureum; Fatty acid; Arachidonic acid; N-stress; #916; 5-desaturase;

    机译：卟啉毒液;脂肪酸;花生酸;n应激;＆＃916;5-去饱和酶;

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