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首页> 外文期刊>Bioprocess and Biosystems Engineering >Controlling filamentous fungi morphology with microparticles to enhanced beta-mannanase production
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Controlling filamentous fungi morphology with microparticles to enhanced beta-mannanase production

机译：用微粒控制丝状真菌的形态以增强β-甘露聚糖酶的产生

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摘要

beta-mannanase was produced mainly by Aspergillus species and can degrade the beta-1,4-mannose linkages of galactomannans. This study was undertaken to enhance mannanase production using talcum and aluminum oxide as the microparticles, which control cell morphology of recombinant Aspergillus sojae in glucose and carob extract medium. Both microparticles improved fungal growth in glucose and carob pod extract medium. Aluminum oxide (1 g/L) was the best agent for glucose medium which resulted in 514.0 U/ml. However, the highest mannanase activity was found as 568.7 U/ml with 5 g/L of talcum in carob extract medium. Increase in microparticle concentration resulted in decreasing the pellet size diameter. Furthermore, more than 10 g/L of talcum addition changed the filamentous fungi growth type from pellet to pellet/mycelium mixture. Results showed that right type and concentration of microparticle in fermentation media improved the mannanase activity and production rate by controlling the growth morphology.
机译：β-甘露聚糖酶主要由曲霉菌种产生，并且可以降解半乳甘露聚糖的β-1,4-甘露糖键。进行本研究以使用滑石粉和氧化铝作为微粒来增强甘露聚糖酶的生产，所述微粒控制葡萄糖和角豆树提取物培养基中重组大豆曲霉的细胞形态。两种微粒均改善了葡萄糖和角豆荚提取物培养基中的真菌生长。氧化铝（1 g / L）是葡萄糖培养基的最佳试剂，其结果为514.0 U / ml。但是，在角豆提取物培养基中，以5 g / L滑石粉发现最高的甘露聚糖酶活性为568.7 U / ml。微粒浓度的增加导致丸粒直径的减小。此外，添加超过10 g / L的滑石粉改变了丝状真菌的生长类型，从颗粒状变为颗粒状/菌丝体混合物。结果表明，正确的类型和浓度的发酵培养基中的微粒可通过控制生长形态来提高甘露聚糖酶活性和生产率。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第9期|1391-1399|共9页
  • 作者

    Yatmaz Ercan; Karahalil Ercan; Germec Mustafa; Ilgin Merve; Turhan Irfan;

  • 作者单位

    Akdeniz Univ, Goynuk Culinary Arts Vocat Sch, TR-07980 Antalya, Turkey|Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey;

    Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey;

    Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey|Cankiri Karatekin Univ, Fac Engn, Dept Food Engn, TR-18100 Cankiri, Turkey;

    Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey;

    Akdeniz Univ, Dept Food Engn, Fac Engn, TR-07058 Antalya, Turkey;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microparticle; beta-mannanase; Aluminum; oxide; Talcum;

    机译：微粒;β-甘露聚糖酶;铝;氧化物;滑石粉;

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